Preprint Article Version 1 This version is not peer-reviewed

Supplementing Conjugated and Non-Conjugated L-Methionine and Acetate Alters Expression Patterns of β-Casein, Proteins, and Metabolites Related to Protein Synthesis in Bovine Mammary Cells

Version 1 : Received: 14 November 2018 / Approved: 15 November 2018 / Online: 15 November 2018 (11:13:55 CET)

How to cite: Conejos, J.R.; Jeon, S.W..; Kim, J.E..; Kim, M.J..; Lee, J.E..; Lee, B.S..; Park, J.S..; Moon, J.O..; Lee, J..; Lee, H. Supplementing Conjugated and Non-Conjugated L-Methionine and Acetate Alters Expression Patterns of β-Casein, Proteins, and Metabolites Related to Protein Synthesis in Bovine Mammary Cells. Preprints 2018, 2018110360 (doi: 10.20944/preprints201811.0360.v1). Conejos, J.R.; Jeon, S.W..; Kim, J.E..; Kim, M.J..; Lee, J.E..; Lee, B.S..; Park, J.S..; Moon, J.O..; Lee, J..; Lee, H. Supplementing Conjugated and Non-Conjugated L-Methionine and Acetate Alters Expression Patterns of β-Casein, Proteins, and Metabolites Related to Protein Synthesis in Bovine Mammary Cells. Preprints 2018, 2018110360 (doi: 10.20944/preprints201811.0360.v1).

Abstract

The study was done to determine the effect of supplementing different forms of L-methionine (L-Met) and acetate on protein synthesis in immortalized bovine mammary epithelial cell line (MAC-T Cell): Control, L-Met, conjugated L-Met and acetate (CMA), and non-conjugated L-Met and Acetate (NMA). Protein synthesis mechanism was determined by omics method. NMA group had the highest protein content in the media and β-casein mRNA expression levels (P < 0.05). The number of upregulated and downregulated proteins observed were 77 and 62 in CMA group and 50 and 81 in NMA group from 448 proteins, respectively (P < 0.05). NMA and CMA treatments stimulated pathways related to protein and energy metabolism (P < 0.05). Metabolomic analysis also revealed CMA and NMA treatments resulted in increases of several metabolites (P < 0.05). In conclusion, NMA treatment increased protein concentration and expression level of β-casein mRNA in MAC-T cells compared to CMA.

Subject Areas

L-methionine, protein synthesis, MAC-T Cell

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