Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Supplementing Conjugated and Non-Conjugated L-Methionine and Acetate Alters Expression Patterns of β-Casein, Proteins, and Metabolites Related to Protein Synthesis in Bovine Mammary Cells

Version 1 : Received: 14 November 2018 / Approved: 15 November 2018 / Online: 15 November 2018 (11:13:55 CET)

How to cite: Conejos, J.R.; Jeon, S.W.; Kim, J.E.; Kim, M.J.; Lee, J.E.; Lee, B.S.; Park, J.S.; Moon, J.O.; Lee, J.; Lee, H. Supplementing Conjugated and Non-Conjugated L-Methionine and Acetate Alters Expression Patterns of β-Casein, Proteins, and Metabolites Related to Protein Synthesis in Bovine Mammary Cells. Preprints 2018, 2018110360. https://doi.org/10.20944/preprints201811.0360.v1 Conejos, J.R.; Jeon, S.W.; Kim, J.E.; Kim, M.J.; Lee, J.E.; Lee, B.S.; Park, J.S.; Moon, J.O.; Lee, J.; Lee, H. Supplementing Conjugated and Non-Conjugated L-Methionine and Acetate Alters Expression Patterns of β-Casein, Proteins, and Metabolites Related to Protein Synthesis in Bovine Mammary Cells. Preprints 2018, 2018110360. https://doi.org/10.20944/preprints201811.0360.v1

Abstract

The study was done to determine the effect of supplementing different forms of L-methionine (L-Met) and acetate on protein synthesis in immortalized bovine mammary epithelial cell line (MAC-T Cell): Control, L-Met, conjugated L-Met and acetate (CMA), and non-conjugated L-Met and Acetate (NMA). Protein synthesis mechanism was determined by omics method. NMA group had the highest protein content in the media and β-casein mRNA expression levels (P < 0.05). The number of upregulated and downregulated proteins observed were 77 and 62 in CMA group and 50 and 81 in NMA group from 448 proteins, respectively (P < 0.05). NMA and CMA treatments stimulated pathways related to protein and energy metabolism (P < 0.05). Metabolomic analysis also revealed CMA and NMA treatments resulted in increases of several metabolites (P < 0.05). In conclusion, NMA treatment increased protein concentration and expression level of β-casein mRNA in MAC-T cells compared to CMA.

Keywords

L-methionine, protein synthesis, MAC-T Cell

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

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