Version 1
: Received: 13 September 2018 / Approved: 13 September 2018 / Online: 13 September 2018 (14:45:35 CEST)
How to cite:
Taguchi, S.; Suga, K.; Hayashi, K.; Okamoto, Y.; Nakamura, H.; Umakoshi, H. Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties. Preprints2018, 2018090242. https://doi.org/10.20944/preprints201809.0242.v1
Taguchi, S.; Suga, K.; Hayashi, K.; Okamoto, Y.; Nakamura, H.; Umakoshi, H. Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties. Preprints 2018, 2018090242. https://doi.org/10.20944/preprints201809.0242.v1
Taguchi, S.; Suga, K.; Hayashi, K.; Okamoto, Y.; Nakamura, H.; Umakoshi, H. Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties. Preprints2018, 2018090242. https://doi.org/10.20944/preprints201809.0242.v1
APA Style
Taguchi, S., Suga, K., Hayashi, K., Okamoto, Y., Nakamura, H., & Umakoshi, H. (2018). Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties. Preprints. https://doi.org/10.20944/preprints201809.0242.v1
Chicago/Turabian Style
Taguchi, S., Hidemi Nakamura and Hiroshi Umakoshi. 2018 "Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties" Preprints. https://doi.org/10.20944/preprints201809.0242.v1
Abstract
Self-assembly membranes, composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), were characterized at the total lipid concentration below 20 mM. The sizes of the assemblies varied depending on the molar ratio of DMPC and DHPC (q = [DMPC]/[DHPC]). The small assemblies with diameter of ca. 10 nm were formed at q ≤ 2.0 at 20 ºC (below phase transition temperature of DMPC). The physicochemical membrane properties were then studied using fluorescence probes, 1,6-diphenyl-1,3,5-hexatriene and 6-dodecanoyl-N,N-dimethyl-2-naphthylamine, upon the dilution. DHPC micelle showed a higher membrane fluidity, while the DMPC/DHPC membranes at q ≥ 0.5 showed lower membrane fluidities as well as DMPC vesicle in gel (ordered) phase. Upon dilution, the ordered membrane properties were maintained while the solution turbidities increased, implying the morphological change of the self-assembly, bicelle to the vesicle in gel phase. Based on the obtained results, a phase diagram of DMPC/DHPC binary system (at 20 ºC) is described: (i) the bicelle suspension is transparent and the membrane is in ordered state, (ii) the micelle suspension is transparent and the membrane is in disordered state, (iii) the vesicle suspension is turbid and the membrane is in ordered state.
Chemistry and Materials Science, Chemical Engineering
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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