Preprint Article Version 1 This version is not peer-reviewed

Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds

Version 1 : Received: 2 September 2018 / Approved: 3 September 2018 / Online: 3 September 2018 (15:00:14 CEST)

A peer-reviewed article of this Preprint also exists.

Jiang, J.-Z.; Kuo, C.-H.; Chen, B.-H.; Chen, M.-K.; Lin, C.-S.; Ho, S.-L. Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds. Int. J. Mol. Sci. 2018, 19, 3247. Jiang, J.-Z.; Kuo, C.-H.; Chen, B.-H.; Chen, M.-K.; Lin, C.-S.; Ho, S.-L. Effects of OsCDPK1 on the Structure and Physicochemical Properties of Starch in Developing Rice Seeds. Int. J. Mol. Sci. 2018, 19, 3247.

Journal reference: Int. J. Mol. Sci. 2018, 19, 3247
DOI: 10.3390/ijms19103247

Abstract

Overexpression of a constitutively active truncated form of OsCDPK1 (OEtr) in rice produced smaller seeds, but a double-stranded RNA gene-silenced form of OsCDPK1 (Ri) yielded large seeds, suggesting that OsCDPK1 plays a functional role in rice seed development. In the study presented here, we propose a model in which OsCDPK1 plays key roles in negatively controlling the grain size, amylose content, and endosperm appearance, and also affects the physicochemical properties of the starch. The dehulled transgenic OEtr grains were smaller than the dehulled wild-type grains, and the OEtr endosperm was opaque and had a low amylose content and numerous small loosely packed polyhedral starch granules. However, the OEtr grain sizes and endosperm appearances were not affected by the temperature being either optimal (25 °C ) or low (22 °C) or high (31 °C) during the grain-filling phase. In contrast, the transgenic Ri grains were larger, had higher amylose contents, and had more transparent endosperms filled with tightly packed polyhedral starch granules. This demonstrates that OsCDPK1 plays a novel functional role in starch biosynthesis during seed development and affects the transparent appearance of the endosperm. These results improve our understanding of the molecular mechanisms through which the grain filling process occurs in rice.

Subject Areas

rice; OsCDPK1; grain size; amylose content; endosperm appearance

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