Preprint Article Version 1 This version not peer reviewed

Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID-A Fully Validated Approach

Version 1 : Received: 27 July 2017 / Approved: 28 July 2017 / Online: 28 July 2017 (13:05:43 CEST)

How to cite: Pasias, I.; Kyriakou, I.; Proestos, C. Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID-A Fully Validated Approach. Preprints 2017, 2017070086 (doi: 10.20944/preprints201707.0086.v1). Pasias, I.; Kyriakou, I.; Proestos, C. Development of a Rapid Method for the Determination of Caffeine in Coffee Grains by GC-FID-A Fully Validated Approach. Preprints 2017, 2017070086 (doi: 10.20944/preprints201707.0086.v1).

Abstract

A simple method for the determination of caffeine in coffee grains by GC-FID is presented in the current work. The method was fully validated according to ISO 17025 requirements and European Commission regulation. The accuracy, as provided by recovery experiments was higher than 93%, and the precision, as provided by the (%)Relative standard deviation under reproducibility conditions, was lower than 5%. All independent parameters that lead in the increase of methods uncertainty were investigated. In the present work all special precautions were taken into account in order to avoid the use of an internal standard. The method was applied in real samples and possible health claims were investigated.

Subject Areas

Caffeine, Uncertainty calculation; ISO 17025, Guatemala coffee grains; GC-FID

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