Preprint Article Version 1 This version not peer reviewed

An on-site, ultra-sensitive, quantitative sensing method for the determination of total aflatoxin in peanut and rice based on quantum dot nanobeads strip

Version 1 : Received: 23 March 2017 / Approved: 24 March 2017 / Online: 24 March 2017 (03:59:06 CET)

A peer-reviewed article of this Preprint also exists.

Ouyang, S.; Zhang, Z.; He, T.; Li, P.; Zhang, Q.; Chen, X.; Wang, D.; Li, H.; Tang, X.; Zhang, W. An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip. Toxins 2017, 9, 137. Ouyang, S.; Zhang, Z.; He, T.; Li, P.; Zhang, Q.; Chen, X.; Wang, D.; Li, H.; Tang, X.; Zhang, W. An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip. Toxins 2017, 9, 137.

Journal reference: Toxins 2017, 9, 137
DOI: 10.3390/toxins9040137

Abstract

An on-site, ultra-sensitive, and quantitative sensing method was developed based on quantum dot nanobeads (QDNBs) and test strip for the determination of total aflatoxins (AFTs) in rice and peanut. The monoclonal antibody against AFT (mAbAFT) was home-made and labeled with QDNB. After the pre-coating of the AFT antigen on the test line (T line), the competitive immunoreactions were conducted between AFT and AFT antigen on the T line with QDNBs-mAbAFT. Under optimal conditions, this approach allowed a rapid response towards AFT with a considerable sensitivity of 1.4 pg/mL and 2.9 pg/mL in rice and peanut matrices, respectively. The put-in and put-out duration were within 10 min. The recoveries for AFT in rice and peanut samples matrices were recorded from 86.25–118.0% with the relative deviations (RSD) below 12%. The assay was further validated via the comparison between this QDNB strip and the conventional HPLC method using spiked samples. Thus, the design provided a potential alternative for on-site, ultra-sensitive, and quantitative sensing of AFT that could also be expanded to other chemical contaminants for food safety.

Subject Areas

total aflatoxin; quantum dot nanobead; test strip; on-site; peanut and rice; agro-food safety

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