Objective: To assess the psychometric properties of the Academic Motivation Scale (AMS) in a sample of university students from Ecuador Method: This instrumental research examines the construct validity through confirmatory factor analysis and the reliability of the AMS. Sample: 1007 subjects (n=403 women, 39.9%) from the Salesian Polytechnic University of Ecuador. Results: Regarding the construct validity of the AMS, confirmatory factor analysis indicated that the model demonstrated a good fit to the data, with CMIN or χ2(303) = 1171.948, p ¡ 0.001, CFI = 0.987 (robust), RMSEA = 0.053 (confidence interval between 0.050 and 0.057), and SRMR = 0.047. The Cronbach’s Alpha (α) and McDonald’s Omega (ω) reliability coefficients were ≥0.80. Conclusion: Given the psychometric properties presented, the use of the AMS-E is recommended for evaluating factors of academic motivation in the Ecuadorian population.