Microplastics (MPs) adsorb hazardous substances and are ingested by a wide range of organisms; therefore, indicators for managing their environmental concentrations are needed. Ideally, threshold values should be based on health impacts. However, the diversity of MPs and the complexity of their environmental behavior make it difficult to establish unified environmental concentration standards. In this study, we propose a threshold for the presence of MPs on sandy beaches based on “visual cleanliness,” derived from the amount of MPs that people find psychologically unacceptable. Three types of MPs were used: white polypropylene (PP), blue PP, and white polystyrene (PS; expanded polystyrene). For defining a narrow-range cleanliness threshold, volume concentration was more appropriate than mass concentration. White particles were expected to be less noticeable because they tended to blend with white shell fragments, which are ubiquitous on beaches. In contrast, blue particles were expected to be less acceptable owing to their rarity. However, we found no difference in unacceptability between white PP and blue PP. The threshold, defined as the volume concentration at which half of the respondents find MPs psychologically unacceptable, ranged from 1 to 2 cm3-MPs/m2-sand. Gender, age, travel time to the beach, and frequency of beach visits did not influence unacceptability. Strong concern about marine plastic pollution and experience in cleaning public spaces were associated with a tendency toward low tolerance for MP contamination on beaches.