The efficient degradation of residues of prometryn is essential as it is widely used to control weeds in wheat fields, but its residues in the soil may lead to a decrease in soil fertility and even affect the growth of subsequent crops. In this experiment, we isolated T6-2 and T7-2 strains, and analyzed the morphological, physiological and biochemical characteristics and 16S rRNA homology of T6-2 and T7-2, and investigated the effects of different pH, inoculum and incubation time on the growth and degradation rate of the strains. The physiological and biochemical characteristics and 16S rRNA homology were used to identify strain T6-2 as Bacillus subtilis subsp. Subtilis and strain T7-2 as Brevundimonas diminuta, with registration numbers MW349862 and MW350067, respectively. The strains T6-2 and T7-2 were annotated by GO, KEGG, COG and CAZyme databases respectively, and predicted to have genes related to herbicide degradation, and the genomic covariance analysis was used to determine the common and unique genes of strains T6-2 and T7-2. This study provides technical support for the remediation of contaminated soil and lays the foundation for research on microbial degradation and bioremediation of contaminated soil.