Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Polyphenols Investigation, Antioxidant and Anticholinesterase Activity of Rosmarinus officinalis L. Species from Southwest Romania Flora

Ludovic Everard Bejenaru
ORCID logo ,
Andrei Biţă
,
George Dan Mogoşanu
* ,
Adina-Elena Segneanu
ORCID logo ,
Antonia Radu
ORCID logo ,
Maria Viorica Ciocîlteu
,
Cornelia Bejenaru
Version 1 : Received: 1 August 2024 / Approved: 2 August 2024 / Online: 2 August 2024 (10:36:53 CEST)

A peer-reviewed article of this Preprint also exists.

Bejenaru, L.E.; Biţă, A.; Mogoşanu, G.D.; Segneanu, A.-E.; Radu, A.; Ciocîlteu, M.V.; Bejenaru, C. Polyphenols Investigation and Antioxidant and Anticholinesterase Activities of Rosmarinus officinalis L. Species from Southwest Romania Flora. Molecules 2024, 29, 4438. Bejenaru, L.E.; Biţă, A.; Mogoşanu, G.D.; Segneanu, A.-E.; Radu, A.; Ciocîlteu, M.V.; Bejenaru, C. Polyphenols Investigation and Antioxidant and Anticholinesterase Activities of Rosmarinus officinalis L. Species from Southwest Romania Flora. Molecules 2024, 29, 4438.

Abstract

Rosemary is one of the most important medicinal plants for natural therapy due to its multiple pharmacological properties, such as antioxidant, anti-inflammatory, neuroprotective, antiproliferative, antitumor, hepato- and nephroprotective, hypolipidemic, hypocholesterolemic, antihypertensive, anti-ischemic, hypoglycemic, radioprotective, antimicrobial, antiviral, antiallergic, wound healing. Our study reports for the first time, over a 12-month period, the identification and quantification of polyphenols and the investigation of the antioxidant and acetylcholinesterase (AChE) inhibitory activity of the Rosmarinus officinalis L. species harvested at flowering from the flora of southwestern Romania (Oltenia Region). Identification and quantification of polyphenolic acids was made by ultra-high-performance liquid chromatography/mass spectrometry (UHPLC/MS). Total phenolic content was determined using the spectrophotometric method. In situ antioxidant and anticholinesterase activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and AChE inhibitory assay, respectively, on high-performance thin-layer chromatography (HPTLC) plates. DPPH radical scavenging activity was also assessed spectrophotometrically. The results revealed significant correlations between specific polyphenolic compounds and the measured biological activities, understanding the role of seasonal variations and providing insights into the optimal harvesting times and medicinal benefits of rosemary. Our research brings new information on the phytochemical profile of R. officinalis, as a natural source of polyphenols with antioxidant and AChE inhibitory properties.

Keywords

Rosmarinus officinalis; Romanian flora; polyphenols; UHPLC/MS quantification; antioxidant activity; anticholinesterase activity

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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