Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications

Gustavo Henrique Doná Rodrigues Almeida; Raquel Souza da Silva; Mariana Sversut Gibin; Victoria Helen de Souza Gonzaga; Henrique dos Santos; Rebeca Piatniczka Igleisa; Leticia Alves Fernandes; Iorrane Couto Fernandes; Thais Naomi Gonçalves Nesiyama; Franciele Sato; Mauro Luciano Baesso; Luzmarina Hernandes; Jaqueline de Carvalho Rinaldi; Flavio Vieira Meirelles; Claudete S. Astolfi-Ferreira; Antonio José Piantino Ferreira; Ana Claudia Oliveira Carreira

doi:10.20944/preprints202405.1629.v1

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preprints.org > biology and life sciences > biology and biotechnology > doi: 10.20944/preprints202405.1629.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications

Gustavo Henrique Doná Rodrigues Almeida

^* ,

Raquel Souza da Silva

Mariana Sversut Gibin

Victoria Helen de Souza Gonzaga

Henrique dos Santos

Rebeca Piatniczka Igleisa

Leticia Alves Fernandes

Iorrane Couto Fernandes

Thais Naomi Gonçalves Nesiyama

Franciele Sato

Mauro Luciano Baesso

Luzmarina Hernandes

Jaqueline de Carvalho Rinaldi

Flavio Vieira Meirelles

Claudete S. Astolfi-Ferreira

Antonio José Piantino Ferreira

Ana Claudia Oliveira Carreira

Version 1 : Received: 24 May 2024 / Approved: 24 May 2024 / Online: 24 May 2024 (11:56:17 CEST)

How to cite: Almeida, G. H. D. R.; da Silva, R. S.; Gibin, M. S.; Gonzaga, V. H. D. S.; dos Santos, H.; Igleisa, R. P.; Fernandes, L. A.; Fernandes, I. C.; Nesiyama, T. N. G.; Sato, F.; Baesso, M. L.; Hernandes, L.; Rinaldi, J. D. C.; Meirelles, F. V.; Astolfi-Ferreira, C. S.; Ferreira, A. J. P.; Carreira, A. C. O. Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications. Preprints 2024, 2024051629. https://doi.org/10.20944/preprints202405.1629.v1 Almeida, G. H. D. R.; da Silva, R. S.; Gibin, M. S.; Gonzaga, V. H. D. S.; dos Santos, H.; Igleisa, R. P.; Fernandes, L. A.; Fernandes, I. C.; Nesiyama, T. N. G.; Sato, F.; Baesso, M. L.; Hernandes, L.; Rinaldi, J. D. C.; Meirelles, F. V.; Astolfi-Ferreira, C. S.; Ferreira, A. J. P.; Carreira, A. C. O. Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications. Preprints 2024, 2024051629. https://doi.org/10.20944/preprints202405.1629.v1

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MDPI and ACS Style

Almeida, G. H. D. R.; da Silva, R. S.; Gibin, M. S.; Gonzaga, V. H. D. S.; dos Santos, H.; Igleisa, R. P.; Fernandes, L. A.; Fernandes, I. C.; Nesiyama, T. N. G.; Sato, F.; Baesso, M. L.; Hernandes, L.; Rinaldi, J. D. C.; Meirelles, F. V.; Astolfi-Ferreira, C. S.; Ferreira, A. J. P.; Carreira, A. C. O. Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications. Preprints 2024, 2024051629. https://doi.org/10.20944/preprints202405.1629.v1

APA Style

Almeida, G. H. D. R., da Silva, R. S., Gibin, M. S., Gonzaga, V. H. D. S., dos Santos, H., Igleisa, R. P., Fernandes, L. A., Fernandes, I. C., Nesiyama, T. N. G., Sato, F., Baesso, M. L., Hernandes, L., Rinaldi, J. D. C., Meirelles, F. V., Astolfi-Ferreira, C. S., Ferreira, A. J. P., & Carreira, A. C. O. (2024). Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications. Preprints. https://doi.org/10.20944/preprints202405.1629.v1

Chicago/Turabian Style

Almeida, G. H. D. R., Antonio José Piantino Ferreira and Ana Claudia Oliveira Carreira. 2024 "Region-Specific Decellularization of Porcine Uterine Tube Extracellular Matrix: A New Approach for Reproductive Tissue-Engineering Applications" Preprints. https://doi.org/10.20944/preprints202405.1629.v1

Abstract

Uterine tube extracellular matrix is a key component that regulates tubal tissue physiology, and it has a region-specific structural distribution, which are directly associated to their functions. Considering this, application of biological matrices in culture systems is an interesting strategy to develop biomimetic tubal microenvironments and enhance their complexity. However, there are no established protocols to produce tubal biological matrices that consider the organ morphophysiology for such applications. Therefore, this study aimed to establish region-specific protocols to obtain decellularized scaffolds derived from porcine infundibulum, ampulla, and isthmus to provide suitable sources of biomaterials for tissue-engineering approaches. Porcine uterine tubes were decellularized in solutions of 0.1% SDS and 0.5% Triton X-100. Decellularization efficiency was evaluated by DAPI staining and DNA quantification. We analyzed ECM composition and structure by optical and scanning electronic microscopy, FTIR and Raman spectroscopy. DNA and DAPI assays validated the decellularization, presenting significative reduction of cellular content. Structural and spectroscopy analyses revealed that produced scaffolds remained well structured and with ECM composition preserved. YS and HEK293 cells were used to attest cytocompatibility, allowing cell survival and successful interaction with the scaffolds. These results suggest that such matrices are applicable for future biotechnological approaches in the reproductive field.

Keywords

uterine tube; extracellular matrix; reproduction; decellularization; biomaterial

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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