Version 1
: Received: 25 April 2024 / Approved: 25 April 2024 / Online: 25 April 2024 (10:13:11 CEST)
How to cite:
Ishii, Y.; Matsunaga, T.; Yasui, T.; Rini, D.M.; Inoue, R.; Yamamoto, Y.; Suzuki, T. Supplemental psyllium fiber increases antimicrobial proteins via the tuft cell-ILC2 circuit and type II immune response in the mouse small intestine. Preprints2024, 2024041657. https://doi.org/10.20944/preprints202404.1657.v1
Ishii, Y.; Matsunaga, T.; Yasui, T.; Rini, D.M.; Inoue, R.; Yamamoto, Y.; Suzuki, T. Supplemental psyllium fiber increases antimicrobial proteins via the tuft cell-ILC2 circuit and type II immune response in the mouse small intestine. Preprints 2024, 2024041657. https://doi.org/10.20944/preprints202404.1657.v1
Ishii, Y.; Matsunaga, T.; Yasui, T.; Rini, D.M.; Inoue, R.; Yamamoto, Y.; Suzuki, T. Supplemental psyllium fiber increases antimicrobial proteins via the tuft cell-ILC2 circuit and type II immune response in the mouse small intestine. Preprints2024, 2024041657. https://doi.org/10.20944/preprints202404.1657.v1
APA Style
Ishii, Y., Matsunaga, T., Yasui, T., Rini, D.M., Inoue, R., Yamamoto, Y., & Suzuki, T. (2024). Supplemental psyllium fiber increases antimicrobial proteins via the tuft cell-ILC2 circuit and type II immune response in the mouse small intestine. Preprints. https://doi.org/10.20944/preprints202404.1657.v1
Chicago/Turabian Style
Ishii, Y., Yoshinari Yamamoto and Takuya Suzuki. 2024 "Supplemental psyllium fiber increases antimicrobial proteins via the tuft cell-ILC2 circuit and type II immune response in the mouse small intestine" Preprints. https://doi.org/10.20944/preprints202404.1657.v1
Abstract
Dietary fibers regulate intestinal barrier function; however, the precise mechanisms remain unclear. This study investigated the effects of psyllium fibers on antimicrobial protein expression, focusing on the type II immunity and tuft cell-group 2 innate lymphoid cell (ILC2) circuit in the small intestine of the mouse. Supplemental psyllium fiber upregulated antimicrobial proteins, such as small proline-rich protein 2A (SPRR2A) and resistin-like beta (RELMβ), in mouse small intestine, evidently affecting cecal microbiota composition. Psyllium fibers also increased the RNA and protein expression of molecules related to ILC2 and tuft cells, such as IL-13, IL-25, DCLK1, Gfi-1b, SH2 domain-containing protein 3C, and Spi-B. In addition, ILC2 inhibitor (disulfiram) and bitter taste receptor blocker administration reduced psyllium-induced SPRR2A and RELMβ expression. Collectively, psyllium supplementation upregulates antimicrobial proteins such as SPRR2A and RELMß via the type II immune response and tuft cell-ILC2 circuit in the mouse small intestine.
Keywords
Antimicrobial protein; Intestinal microbiota; Mice; Psyllium fiber; Type II immunity; Tuft cells
Subject
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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