Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells

José Luis González-Quiroz; Juan Moisés Ocampo-Godínez; Victoria Noemi Hernández-González; Ruth Angélica Lezama; Elba Reyes-Maldonado; Armando Vega-López; María Lilia Domínguez-López

doi:10.20944/preprints202404.0934.v1

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preprints.org > medicine and pharmacology > oncology and oncogenics > doi: 10.20944/preprints202404.0934.v1

Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells

José Luis González-Quiroz

Juan Moisés Ocampo-Godínez

Victoria Noemi Hernández-González

Ruth Angélica Lezama

Elba Reyes-Maldonado

Armando Vega-López

María Lilia Domínguez-López

Version 1 : Received: 13 April 2024 / Approved: 15 April 2024 / Online: 15 April 2024 (10:20:48 CEST)

How to cite: González-Quiroz, J.L.; Ocampo-Godínez, J.M.; Hernández-González, V.N.; Lezama, R.A.; Reyes-Maldonado, E.; Vega-López, A.; Domínguez-López, M.L. Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells. Preprints 2024, 2024040934. https://doi.org/10.20944/preprints202404.0934.v1 González-Quiroz, J.L.; Ocampo-Godínez, J.M.; Hernández-González, V.N.; Lezama, R.A.; Reyes-Maldonado, E.; Vega-López, A.; Domínguez-López, M.L. Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells. Preprints 2024, 2024040934. https://doi.org/10.20944/preprints202404.0934.v1

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MDPI and ACS Style

González-Quiroz, J.L.; Ocampo-Godínez, J.M.; Hernández-González, V.N.; Lezama, R.A.; Reyes-Maldonado, E.; Vega-López, A.; Domínguez-López, M.L. Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells. Preprints 2024, 2024040934. https://doi.org/10.20944/preprints202404.0934.v1

APA Style

González-Quiroz, J.L., Ocampo-Godínez, J.M., Hernández-González, V.N., Lezama, R.A., Reyes-Maldonado, E., Vega-López, A., & Domínguez-López, M.L. (2024). Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells. Preprints. https://doi.org/10.20944/preprints202404.0934.v1

Chicago/Turabian Style

González-Quiroz, J.L., Armando Vega-López and María Lilia Domínguez-López. 2024 "Pentoxifylline and Norcantharidin Modify p62 Expression in 2D and 3D Cultures of B16F1 Cells" Preprints. https://doi.org/10.20944/preprints202404.0934.v1

Abstract

3D cell cultures have improved the evaluation of drugs for cancer therapy, due to their high similarity to solid tumors. In melanoma, autophagy appears to show a dual role depending on the progression of the disease. p62 protein has been proposed for the evaluation of autophagic flux since its expression is an indicator of the state of autophagy. Pentoxifylline (PTX) and Norcantharidin (NCTD) are drugs that have been shown to have anticancer effects in various types of cancer. In this work, B16F1 mouse melanoma cell line was used, from which two-dimensional (2D) monolayer cultures and three-dimensional (3D) organoids were carried out using the hanging drop technique. The expression of p62 was determined by western blot and immunofluorescences. Our results indicate that pentoxifylline reduces p62 expression in both types of culture. While Norcantharidin increases its expression in 3D cultures at 24 h. Therefore, these drugs could have a potential therapeutic use for the regulation of autophagy in melanoma, depending on the state of evolution of the disease.

Keywords

Melanoma; 3D cultures; autophagy; p62; spheroids

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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