Version 1
: Received: 11 April 2024 / Approved: 12 April 2024 / Online: 12 April 2024 (09:52:23 CEST)
How to cite:
Barros, S.C.I.M.; Figueira, A.D.R.; Silveira, A.T.L. Molecular Characterization of Isolates of the Banana bunchy top virus from the District of Chókwè, Mozambique. Preprints2024, 2024040838. https://doi.org/10.20944/preprints202404.0838.v1
Barros, S.C.I.M.; Figueira, A.D.R.; Silveira, A.T.L. Molecular Characterization of Isolates of the Banana bunchy top virus from the District of Chókwè, Mozambique. Preprints 2024, 2024040838. https://doi.org/10.20944/preprints202404.0838.v1
Barros, S.C.I.M.; Figueira, A.D.R.; Silveira, A.T.L. Molecular Characterization of Isolates of the Banana bunchy top virus from the District of Chókwè, Mozambique. Preprints2024, 2024040838. https://doi.org/10.20944/preprints202404.0838.v1
APA Style
Barros, S.C.I.M., Figueira, A.D.R., & Silveira, A.T.L. (2024). Molecular Characterization of Isolates of the <em>Banana bunchy top virus</em> from the District of Chókwè, Mozambique. Preprints. https://doi.org/10.20944/preprints202404.0838.v1
Chicago/Turabian Style
Barros, S.C.I.M., Antonia dos Reis Figueira and Antonia Thalyta Lopes Silveira. 2024 "Molecular Characterization of Isolates of the <em>Banana bunchy top virus</em> from the District of Chókwè, Mozambique" Preprints. https://doi.org/10.20944/preprints202404.0838.v1
Abstract
Banana bunchy top virus (BBTV) was recently detected in Mozambique and appears to be limited to the provinces of Gaza, Maputo and Zambezia, but it has great potential to spread to other provinces. Despite its importance, nothing is known about the BBTV isolates that occur in Mozambique. In this study, the sequences of the S and R genes of 40 isolates chosen as representatives of samples collected previously from eleven farms of the four administrative posts of the district of Chóckwè, Gaza province, were sequenced and analyzed. The S-DNA nucleotide sequences of the analyzed isolates were highly conserved, with identity ranging from 97% to 100%. The same was observed for the R-DNA sequences, with most identities ranging between 98% and 100% among the isolates from Chókwè and above 90% when compared to the isolates from GenBank. The phylogenetic analysis showed that the Mozambican BBTV isolates belong to the Pacific-Indian Oceans (PIO) group, showing greater proximity to the isolate JQ820453 from Malawi than to the isolates from sub-Saharan countries, which were grouped in a distinct subclade. This is the first study conducted to determine the molecular characteristics of BBTV isolates present in Mozambique.
Keywords
nanovirus; Pacific-Indian Oceans (PIO); South-east Asian (SEA); Phylogeny; DNA S; DNA R; Sub-Saharan African (SSA)
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
