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The Effect of Orally Administered Multi-Strain Probiotic Formulation (Lactobacillus, Bifidobacterium) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs
Version 1
: Received: 6 April 2024 / Approved: 8 April 2024 / Online: 8 April 2024 (08:41:32 CEST)
How to cite:
Wójcik, R.; Małaczewska, J.; Tobolski, D.; Miciński, J.; Kaczorek-Łukowska, E.; Zwierzchowski, G. The Effect of Orally Administered Multi-Strain Probiotic Formulation (Lactobacillus, Bifidobacterium) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs. Preprints2024, 2024040507. https://doi.org/10.20944/preprints202404.0507.v1
Wójcik, R.; Małaczewska, J.; Tobolski, D.; Miciński, J.; Kaczorek-Łukowska, E.; Zwierzchowski, G. The Effect of Orally Administered Multi-Strain Probiotic Formulation (Lactobacillus, Bifidobacterium) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs. Preprints 2024, 2024040507. https://doi.org/10.20944/preprints202404.0507.v1
Wójcik, R.; Małaczewska, J.; Tobolski, D.; Miciński, J.; Kaczorek-Łukowska, E.; Zwierzchowski, G. The Effect of Orally Administered Multi-Strain Probiotic Formulation (Lactobacillus, Bifidobacterium) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs. Preprints2024, 2024040507. https://doi.org/10.20944/preprints202404.0507.v1
APA Style
Wójcik, R., Małaczewska, J., Tobolski, D., Miciński, J., Kaczorek-Łukowska, E., & Zwierzchowski, G. (2024). The Effect of Orally Administered Multi-Strain Probiotic Formulation (<em>Lactobacillus</em>, <em>Bifidobacterium</em>) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs. Preprints. https://doi.org/10.20944/preprints202404.0507.v1
Chicago/Turabian Style
Wójcik, R., Edyta Kaczorek-Łukowska and Grzegorz Zwierzchowski. 2024 "The Effect of Orally Administered Multi-Strain Probiotic Formulation (<em>Lactobacillus</em>, <em>Bifidobacterium</em>) on the Phagocytic Activity and Oxidative Metabolism of Peripheral Blood Granulocytes and Monocytes in Lambs" Preprints. https://doi.org/10.20944/preprints202404.0507.v1
Abstract
Probiotic feed additives have attracted considerable research interest in recent years because the effectiveness of probiotics can differ across microbial strains and the supplemented macroorganisms. The present study was conducted on 16 lambs divided into two groups (C - control and E - experimental). The examined lambs were aged 11 days at the beginning of the experiment and 40 days at the end of the experiment. The diet of group E lambs was supplemented with a multi-strain probiotic formulation (Lactobacillus plantarum AMT14, Lactobacillus plantarum AMT4, Lactobacillus rhamnosus AMT15, and Bifidobacterium animalis AMT30), whereas group C lambs did not receive the probiotic additive. At the beginning of the experiment (day 0) and on experimental days 15 and 30, blood was sampled from the jugular vein to determine and compare: phagocytic activity (PHAGOTEST) and oxidative metabolism (PHAGOBURST) of peripheral blood granulocytes and monocytes by flow cytometry. An analysis of the phagocytic activity of granulocytes and monocytes revealed significantly higher levels of phagocytic activity (expressed as the percentage of phagocytic cells and mean fluorescence intensity) in lambs administered the multi-strain probiotic formulation than in the control group. The probiotic feed additive also exerted a positive effect on the oxidative metabolism of both granulocytes and monocytes (expressed as the percentage of oxidative metabolism and mean fluorescence intensity) after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate).
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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