Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches

Peter James Icalia Gann; Jimmbeth Zenila P. Fabia; Althea Gay B. Pagurayan; Ma. Joy Theresa Agcaoili; Ryan James J. Pascual; Suerte M. Baranda; Arc Josam J. Racho; Marvielyn P. Olivar; Jayson F. Cariaga; Alvin Domingo; Dionisio S. Bucao; Shirley C. Agrupis

doi:10.20944/preprints202404.0184.v1

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preprints.org > biology and life sciences > biochemistry and molecular biology > doi: 10.20944/preprints202404.0184.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches

Peter James Icalia Gann

^* ,

Jimmbeth Zenila P. Fabia

Althea Gay B. Pagurayan

Ma. Joy Theresa Agcaoili

Ryan James J. Pascual

Version 1 : Received: 1 April 2024 / Approved: 1 April 2024 / Online: 2 April 2024 (10:16:40 CEST)

How to cite: Gann, P.J.I.; Fabia, J.Z.P.; Pagurayan, A.G.B.; Agcaoili, M.J.T.; Pascual, R.J.J.; Baranda, S.M.; Racho, A.J.J.; Olivar, M.P.; Cariaga, J.F.; Domingo, A.; Bucao, D.S.; Agrupis, S.C. Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches. Preprints 2024, 2024040184. https://doi.org/10.20944/preprints202404.0184.v1 Gann, P.J.I.; Fabia, J.Z.P.; Pagurayan, A.G.B.; Agcaoili, M.J.T.; Pascual, R.J.J.; Baranda, S.M.; Racho, A.J.J.; Olivar, M.P.; Cariaga, J.F.; Domingo, A.; Bucao, D.S.; Agrupis, S.C. Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches. Preprints 2024, 2024040184. https://doi.org/10.20944/preprints202404.0184.v1

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MDPI and ACS Style

Gann, P.J.I.; Fabia, J.Z.P.; Pagurayan, A.G.B.; Agcaoili, M.J.T.; Pascual, R.J.J.; Baranda, S.M.; Racho, A.J.J.; Olivar, M.P.; Cariaga, J.F.; Domingo, A.; Bucao, D.S.; Agrupis, S.C. Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches. Preprints 2024, 2024040184. https://doi.org/10.20944/preprints202404.0184.v1

APA Style

Gann, P.J.I., Fabia, J.Z.P., Pagurayan, A.G.B., Agcaoili, M.J.T., Pascual, R.J.J., Baranda, S.M., Racho, A.J.J., Olivar, M.P., Cariaga, J.F., Domingo, A., Bucao, D.S., & Agrupis, S.C. (2024). Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches. Preprints. https://doi.org/10.20944/preprints202404.0184.v1

Chicago/Turabian Style

Gann, P.J.I., Dionisio S. Bucao and Shirley C. Agrupis. 2024 "Carbohydrate Hydrolytic Activity, Antibiotic Resistance and Stress Tolerance of Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54 for Probiotics Using Genomic and Biochemical Approaches" Preprints. https://doi.org/10.20944/preprints202404.0184.v1

Abstract

Probiotics are microorganisms infused in products for health benefits including acceleration of nutrient digestion, however, it is also important to ensure the safety prior to incorporation. Here, we present evidence of the ability of two probiotic isolates,Lacticaseibacillus paracasei BCRC-16100 and Lacticaseibacillus paracasei ZFM54, in the (1) enhancement of carbohydrate digestion, (2) tolerance to stress, and (3) antibiotic resistance. Approaches include whole genome sequence (WGS) analysis and bioactivity assays. WGS revealed genes suggesting the ability of the two isolates to promote carbohydrate digestion, tolerance to stress and antibiotic resistance. Carbohydrate digestive ability was confirmed through a biochemical assay where the two isolates cause glucose release from starch. The two isolates also showed versatility in a range of temperature and alcohol concentration. For antibiotic resistance particularly on vancomycin, there are three mechanisms namely transporter control, transcriptional regulation, and efflux pump. Furthermore, promoter and transposable element analysis showed that some of the active antibiotic resistant (AbR) genes can be laterally transferred. Altogether, we show the potential of two probiotic isolates to be incorporated in products for improved carbohydrate digestion and the need to address the removal of active and mobile antibiotic resistance genes that may compromise safety.

Keywords

probiotics; nutrient digestion; antibiotic resistance; antibacterial activity; whole genome sequence

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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