Walterová, L.; Botella, L.; Hejna, O.; de la Peña, M.; Tonka, T.; Čurn, V. Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic. Viruses2024, 16, 610.
Walterová, L.; Botella, L.; Hejna, O.; de la Peña, M.; Tonka, T.; Čurn, V. Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic. Viruses 2024, 16, 610.
Walterová, L.; Botella, L.; Hejna, O.; de la Peña, M.; Tonka, T.; Čurn, V. Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic. Viruses2024, 16, 610.
Walterová, L.; Botella, L.; Hejna, O.; de la Peña, M.; Tonka, T.; Čurn, V. Characterization of Mycoviruses in Armillaria ostoyae and A. cepistipes in the Czech Republic. Viruses 2024, 16, 610.
Abstract
Members of the genus Armillaria are widespread forest pathogens against which effective protection has not yet been developed. Due to their longevity and the creation of large-scale cloning of Armillaria individuals, the use of mycoviruses as biocontrol agents (BCAs) against these pathogens could be an effective alternative. This work describes the screening of double-stranded RNA banding patterns as a first approach for potential virus discovery and the use of stranded total RNA-seq for viral detection and characterization in Armillaria spp. collected in the Czech Republic. A total of five single-stranded RNA viruses were detected in Armillaria ostoyae and A. cepistipes, including the first virus of the family Tymoviridae found infecting a member the genus Armillaria and four viruses belonging to the recently described virus group "ambiviruses" with circular ambisense genome organization. Both hammerhead (HHRz) and hairpin (HpRz) ribozymes were detected in all the ambiviricot sequences. Armillaria viruses were compared by phylogenetic analysis and confirmed their specific host by direct RT-PCR, which showed the occurrence of interspecies transmission.
Keywords
Root rot, viruses; circular genetic elements; ambivirus; tymovirus; biological control
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
