Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Cultivation of Bovine MSCs on Plant-Based Scaffolds in a macrofluidic Single-Use Bioreactor for Cultured Meat

Gilad Gome
* ORCID logo ,
Benyamin Chak
,
Shadi Tawil
,
Dafna Shpatz
,
Jonathan Giron
ORCID logo ,
Ilan Brajzblat
,
Chen Weizman
,
Andrey Grishko
,
Sharon Schlesinger
* ORCID logo ,
Oded Shoseyov
*
Version 1 : Received: 21 March 2024 / Approved: 22 March 2024 / Online: 26 March 2024 (10:53:58 CET)

How to cite: Gome, G.; Chak, B.; Tawil, S.; Shpatz, D.; Giron, J.; Brajzblat, I.; Weizman, C.; Grishko, A.; Schlesinger, S.; Shoseyov, O. Cultivation of Bovine MSCs on Plant-Based Scaffolds in a macrofluidic Single-Use Bioreactor for Cultured Meat. Preprints 2024, 2024031587. https://doi.org/10.20944/preprints202403.1587.v1 Gome, G.; Chak, B.; Tawil, S.; Shpatz, D.; Giron, J.; Brajzblat, I.; Weizman, C.; Grishko, A.; Schlesinger, S.; Shoseyov, O. Cultivation of Bovine MSCs on Plant-Based Scaffolds in a macrofluidic Single-Use Bioreactor for Cultured Meat. Preprints 2024, 2024031587. https://doi.org/10.20944/preprints202403.1587.v1

Abstract

One of the main challenges of cultured meat is reducing the costs of production, often described as scaling. The main components of the cultivation process include: cells, media, scaffolds and bioreactors. This work presents an alternative to current stainless steel or glass bioreactors and uses food-grade plant-based scaffolds. Bioreactors must be sterile and leak-free to maintain a proper environment for cell growth. Thermoplastic films are a popular material for constructing these fluidic systems, but traditional methods of welding these film are expensive and do not allow rapid prototyping resulting in high research and development costs for companies. In this study, a laser welding method was developed to join thermoplastic films in a way that prevents contamination and leaks, affording effective macro-fluidics fabrication. This technique was tested using polyethylene (PET) films and a laser cutter operating with settings calibrated for the material to be welded or cut. The laser welding method was found to produce strong, leak-free seals in PET films with minimal heat-induced damage to the films. The ability to design fluidics, chambers and ports of various size. Using this method afforded the incorporation of plant-based scaffolds from food-grade plants (Rice). The laser welding method developed in this study provides a reliable, contamination-free method for the rapid fabrication of fluidic systems for cell cultivation. This technique has the potential to be compatible with a vast range of cell types and scaffolds and to be widely adopted in tissue engineering for regenerative medicine and food applications such as cultured meat.

Keywords

fluidics; bioreactors; tissue engineering; cultured meat; scaffolds

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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