Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

ProAKAP4 as Indicator of Post-thaw Motility in Stallion

Marta DORDAS-PERPINYÀ
,
Ivan Yanez-Ortiz
ORCID logo ,
Nicolas Sergeant
,
Vincent Mevel
,
Jaime Catalán Bahamondes
ORCID logo ,
Jean François Bruyas
,
JORDI MIRO
* ORCID logo ,
Lamia Briand-Amirat
* ORCID logo
Version 1 : Received: 22 March 2024 / Approved: 22 March 2024 / Online: 25 March 2024 (07:54:22 CET)

A peer-reviewed article of this Preprint also exists.

Dordas-Perpinyà, M.; Yánez-Ortiz, I.; Sergeant, N.; Mevel, V.; Catalán, J.; Bruyas, J.-F.; Miró, J.; Briand-Amirat, L. ProAKAP4 as Indicator of Long-Lasting Motility Marker in Post-Thaw Conditions in Stallions. Animals 2024, 14, 1264. Dordas-Perpinyà, M.; Yánez-Ortiz, I.; Sergeant, N.; Mevel, V.; Catalán, J.; Bruyas, J.-F.; Miró, J.; Briand-Amirat, L. ProAKAP4 as Indicator of Long-Lasting Motility Marker in Post-Thaw Conditions in Stallions. Animals 2024, 14, 1264.

Abstract

ProAKAP4, a precursor of AKAP4 (A-kinase anchor protein) found in the flagellum of mammalian and non-mammalian spermatozoa, serves as a structural protein with established correlations to motility parameters across diverse species. This study aimed to determine the proAKAP4 levels evolution in thawed stallion semen over a 3-hour period, examining its correlation with motility descriptors and mitochondrial membrane potential. Utilizing sixteen ejaculates from four French warmblood stallions, the study involved maintaining thawed samples at 37°C for 3 hours, conducting proAKAP4 enzyme-linked immunosorbent assay (ELISA), computer-assisted sperm analysis (CASA), and mitochondrial membrane potential by JC-1 probe and flow cytometry at 0, 1, and 3 hours post-thawing. The findings indicate significant positive correlations (p≤0.05) between proAKAP4 levels and sperm total or progressive motility at all time points analyzed. Spermatozoa velocity descriptors (VAP, VCL, VSL) and spermatozoa lateral head displacement (ALH) display positive correlations (p≤0.05) with ProAKAP4 at the 0-hour post-thawing. ProAKAP4 concentration exhibits no discernible difference between batches with or without cryoprotectant. Notably, proAKAP4 consumption remains insignificant within the initial hour after thawing but becomes significant (p≤0.05) between 1 and 3 hours post-thawing. In summary, proAKAP4 demonstrates positive correlations with total and progressive motility in stallion semen for up to 3 hours after thawing, albeit showing a noticeable decrease starting from the first hour post-thawing, indicating a progressive consumption as a result of spermatozoa motile activity.

Keywords

ProAKAP4, AKAP4, stallion, spermatozoa, motility, mitochondrial activity.

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.