PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography
Version 1
: Received: 13 March 2024 / Approved: 13 March 2024 / Online: 14 March 2024 (08:01:30 CET)
How to cite:
Urbańska, M. Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography. Preprints2024, 2024030796. https://doi.org/10.20944/preprints202403.0796.v1
Urbańska, M. Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography. Preprints 2024, 2024030796. https://doi.org/10.20944/preprints202403.0796.v1
Urbańska, M. Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography. Preprints2024, 2024030796. https://doi.org/10.20944/preprints202403.0796.v1
APA Style
Urbańska, M. (2024). Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography. Preprints. https://doi.org/10.20944/preprints202403.0796.v1
Chicago/Turabian Style
Urbańska, M. 2024 "Application of Polysaccharide‐Based Columns for the Separation of Racemic Mixtures Obtained Based on (R,S)‐2‐Octanol by Reversed‐Phase Chiral Liquid Chromatography" Preprints. https://doi.org/10.20944/preprints202403.0796.v1
Abstract
The polysaccharide-based chiral stationary phase (CSP) columns for high-performance liquid chromatography (HPLC) were applied in the separation of liquid crystalline racemic mixtures obtained based on (R,S)-2-octanol and were found to be highly effective in recognizing isomers of minor structural differences. The mobile phase (MP), which consists of acetonitrile (ACN)/water (H2O) at different volume ratios, was used. All measurements were made at room temperature. The order of the elution was also determined. The chromatographic parameters such as resolution - (Rs), selectivity - (α), and the number of theoretical plates, i.e., column efficiency - (N), were determined. The polysaccharide-based CSP columns have unique advantages in separation technology, and this study has shown the potential usefulness of the CSP columns in separating liquid crystalline racemic mixtures belonging to the same homologous series.
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
