Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Identification and Prevalence of the Mite Carpoglyphus lactis (Acarina: Carpoglyphidae) in Apis mellifera in the Republic of Korea

Thi-Thu Nguyen
ORCID logo ,
Mi-Sun Yoo
,
Hyang-Sim Lee
,
So Youn Youn
,
Se-Ji Lee
,
Su-Kyoung Seo
,
Jaemyung Kim
,
Yun Sang Cho
* ORCID logo
Version 1 : Received: 13 March 2024 / Approved: 13 March 2024 / Online: 13 March 2024 (16:10:18 CET)

A peer-reviewed article of this Preprint also exists.

Nguyen, T.-T.; Yoo, M.-S.; Lee, H.-S.; Youn, S.-Y.; Lee, S.-J.; Seo, S.-K.; Kim, J.-Y.; Cho, Y.-S. Molecular Identification and Prevalence of the Mite Carpoglyphus lactis (Acarina: Carpoglyphidae) in Apis mellifera in the Republic of Korea. Insects 2024, 15, 271. Nguyen, T.-T.; Yoo, M.-S.; Lee, H.-S.; Youn, S.-Y.; Lee, S.-J.; Seo, S.-K.; Kim, J.-Y.; Cho, Y.-S. Molecular Identification and Prevalence of the Mite Carpoglyphus lactis (Acarina: Carpoglyphidae) in Apis mellifera in the Republic of Korea. Insects 2024, 15, 271.

Abstract

Apis mellifera, especially weak ones, are highly vulnerable to Carpoglyphus lactis mites, which can rapidly infest and consume stored pollen, leading to weakened colonies and potentially colony collapse. This study aimed to ascertain and investigate the prevalence of this mite in honeybee colonies across 9 provinces in the Republic of Korea (ROK). A total of 615 honeybee colonies were collected, from 66 apiaries during the spring and 58 apiaries during the summer of 2023. A 1,242 bp segment of the the Cytochrome c oxidase subunit 1 (COI) gene was amplified using the polymerase chain reaction method. The detection levels of C. lactis in the honeybees were compared between winter and summer. Based on the COI sequence analysis, the nucleotide sequence similarity of C. lactis mites isolated in the ROK with those from China (NC048990.1) was found to be 99.5%, and with those from the United Kingdom (KY922482.1) was 99.3%. This study is the first report of C. lactis in Korean apiaries. The findings in this study demonstrate a significantly higher detection rate in winter, which is 4.1 times greater than that in summer (p < 0.001). Furthermore, the results underscore the usefulness of molecular diagnostic techniques for detecting C. lactis mites.

Keywords

Apis mellifera; Carpoglyphus lactis; Cytochrome c oxidase subunit 1 (COI) gene; Molecular identification; Republic of Korea

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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