PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Familial Alzheimer’s Disease Neurons Bearing Mutations in PSEN1 Display Increased Calcium Responses to AMPA as an Early Calcium Dysregulation Phenotype
Version 1
: Received: 29 February 2024 / Approved: 29 February 2024 / Online: 29 February 2024 (12:58:21 CET)
How to cite:
Targa Dias Anastacio, H.; Matosin, N.; Ooi, L. Familial Alzheimer’s Disease Neurons Bearing Mutations in PSEN1 Display Increased Calcium Responses to AMPA as an Early Calcium Dysregulation Phenotype. Preprints2024, 2024021721. https://doi.org/10.20944/preprints202402.1721.v1
Targa Dias Anastacio, H.; Matosin, N.; Ooi, L. Familial Alzheimer’s Disease Neurons Bearing Mutations in PSEN1 Display Increased Calcium Responses to AMPA as an Early Calcium Dysregulation Phenotype. Preprints 2024, 2024021721. https://doi.org/10.20944/preprints202402.1721.v1
Targa Dias Anastacio, H.; Matosin, N.; Ooi, L. Familial Alzheimer’s Disease Neurons Bearing Mutations in PSEN1 Display Increased Calcium Responses to AMPA as an Early Calcium Dysregulation Phenotype. Preprints2024, 2024021721. https://doi.org/10.20944/preprints202402.1721.v1
APA Style
Targa Dias Anastacio, H., Matosin, N., & Ooi, L. (2024). Familial Alzheimer’s Disease Neurons Bearing Mutations in PSEN1 Display Increased Calcium Responses to AMPA as an Early Calcium Dysregulation Phenotype. Preprints. https://doi.org/10.20944/preprints202402.1721.v1
Chicago/Turabian Style
Targa Dias Anastacio, H., Natalie Matosin and Lezanne Ooi. 2024 "Familial Alzheimer’s Disease Neurons Bearing Mutations in PSEN1 Display Increased Calcium Responses to AMPA as an Early Calcium Dysregulation Phenotype" Preprints. https://doi.org/10.20944/preprints202402.1721.v1
Abstract
Familial Alzheimer’s disease (FAD) can be caused by mutations in PSEN1 that encodes presenilin-1, a component of the gamma-secretase complex that cleaves amyloid precursor protein. Alterations in calcium (Ca2+) homeostasis and glutamate signaling are implicated in the pathogenesis of FAD, however it has been difficult to assess in humans whether or not these phenotypes are the result of amyloid or tau pathology. This study aimed to assess early calcium and glutamate phenotypes of FAD by measuring the Ca2+ response of induced pluripotent stem cell (iPSC)-derived neurons bearing PSEN1 mutations to glutamate and the ionotropic glutamate receptor agonists NMDA, AMPA and kainate compared to isogenic control and healthy lines. The data shows that in early neurons, even in the absence of amyloid and tau phenotypes, FAD neurons exhibit increased Ca2+ responses to glutamate and AMPA, but not NMDA or kainate. Together this suggests that PSEN1 mutations alter Ca2+ and glutamate signaling as an early phenotype of FAD.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
