Version 1
: Received: 23 February 2024 / Approved: 23 February 2024 / Online: 23 February 2024 (11:21:02 CET)
How to cite:
Aloysius, M.T.; Felekkis, K.; Petrou, C.C.; Egwim, E.C.; Andreou, E. In Vitro Glucose Uptake in Yeast Cell Facilitated by Abelmoschus esculentus L. (Okra Seed) for Management of Type 2 Diabetes. Preprints2024, 2024021376. https://doi.org/10.20944/preprints202402.1376.v1
Aloysius, M.T.; Felekkis, K.; Petrou, C.C.; Egwim, E.C.; Andreou, E. In Vitro Glucose Uptake in Yeast Cell Facilitated by Abelmoschus esculentus L. (Okra Seed) for Management of Type 2 Diabetes. Preprints 2024, 2024021376. https://doi.org/10.20944/preprints202402.1376.v1
Aloysius, M.T.; Felekkis, K.; Petrou, C.C.; Egwim, E.C.; Andreou, E. In Vitro Glucose Uptake in Yeast Cell Facilitated by Abelmoschus esculentus L. (Okra Seed) for Management of Type 2 Diabetes. Preprints2024, 2024021376. https://doi.org/10.20944/preprints202402.1376.v1
APA Style
Aloysius, M.T., Felekkis, K., Petrou, C.C., Egwim, E.C., & Andreou, E. (2024). In Vitro Glucose Uptake in Yeast Cell Facilitated by Abelmoschus esculentus L. (Okra Seed) for Management of Type 2 Diabetes. Preprints. https://doi.org/10.20944/preprints202402.1376.v1
Chicago/Turabian Style
Aloysius, M.T., Evans Chidi Egwim and Eleni Andreou. 2024 "In Vitro Glucose Uptake in Yeast Cell Facilitated by Abelmoschus esculentus L. (Okra Seed) for Management of Type 2 Diabetes" Preprints. https://doi.org/10.20944/preprints202402.1376.v1
Abstract
In vitro glucose uptake in yeast cell facilitated by Abelmoschus esculentus L. (okra seed) for management of type 2 diabetes was studied. Plant material was collected, identified, processed, and stored for further use. 80% methanol was employed for extraction and sonicated to release antidiabetic-bioactive component in solution and was filtered, concentrated, freeze-dried, and fractionated using standard techniques. Glucose uptake at an initial concentration of 5mM/L and 10mM/L by the crude extract was consistent to that of the known standard drug while at 25mM/L glucose concentration was equivalent with the crude extract. Also, at 0.625 mg/mL the linear equations, and R2 demonstrations that the crude extract was higher in dose predictability than the standard drug as presented by the equation; y = 35.754x - 57.822, and R² = 0.9502 (95%). The extract-fractions were employed to evaluate the ability of yeast cell line culture to take up glucose from the system through DPPH, FRAP, lipid peroxidation and anti-diabetes effect of extract-fraction assays. Extract-fractions were found to poses antioxidant activity high enough to inhibit stress-related diseases. The extract fractions were active as drug candidates both at low and high concentrations and were better compared with the standard drug and standard antioxidant was comparable. The high bioactive extract fractions require encapsulation with a nanoparticle as a drug candidate for type 2 diabetes patients. And an animal trial of the drug candidate may be necessary to monitor the in vivo performances of the extract fractions and subsequent human volunteered trial.
Keywords
keyword Glucose uptake, Yeast cell line, Abelmoschus esculentus L., Extract fractions, Type 2 diabetes, drug candidate.
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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