Version 1
: Received: 21 February 2024 / Approved: 22 February 2024 / Online: 22 February 2024 (07:49:33 CET)
How to cite:
Zeng, H.; Huang, B.; XU, L.; Wu, Y. Banana Classification Using Sanger Sequencing of the Ribosomal DNA Internal Transcribed Spacers (ITS). Preprints2024, 2024021260. https://doi.org/10.20944/preprints202402.1260.v1
Zeng, H.; Huang, B.; XU, L.; Wu, Y. Banana Classification Using Sanger Sequencing of the Ribosomal DNA Internal Transcribed Spacers (ITS). Preprints 2024, 2024021260. https://doi.org/10.20944/preprints202402.1260.v1
Zeng, H.; Huang, B.; XU, L.; Wu, Y. Banana Classification Using Sanger Sequencing of the Ribosomal DNA Internal Transcribed Spacers (ITS). Preprints2024, 2024021260. https://doi.org/10.20944/preprints202402.1260.v1
APA Style
Zeng, H., Huang, B., XU, L., & Wu, Y. (2024). Banana Classification Using Sanger Sequencing of the Ribosomal DNA Internal Transcribed Spacers (ITS). Preprints. https://doi.org/10.20944/preprints202402.1260.v1
Chicago/Turabian Style
Zeng, H., Linbing XU and Yuanli Wu. 2024 "Banana Classification Using Sanger Sequencing of the Ribosomal DNA Internal Transcribed Spacers (ITS)" Preprints. https://doi.org/10.20944/preprints202402.1260.v1
Abstract
Many types of banana (Musa spp.)—one of the most economically important horticultural crops—exist, and their ploidy (diploid, triploid, and tetraploid, usually) and genome types (containing A or/and B genome in most cases) differ. At present, observation and genome type detection are commonly used to identify banana germplasm resources. However, the former is tedious, while the latter cannot distinguish categories below genome types. It is therefore urgent that a simple and effective method for identifying banana germplasm resources is established. We sequenced and analyzed the ribosomal DNA internal transcribed spacer (ITS) sequences of 62 banana germplasm and found that the sequencing peaks, especially the 20 bp region near the 420 bp position (referred to as the “420 bp region”), exhibited relatively recognizable and repeatable polymorphism characteristics. Using the “420 bp region” as a marker, we were able to quickly distinguish bananas belonging to different genome type groups (comprising the AA, AB, AAA, ABB, AAB, ABBB, AAAB, and AAAA groups) or different subgroups in the same genome type group (for example, the Cavendish, Gros Michel, Red, Lakatan, and Ibota Bota subgroups in the AAA group; and the Pisang Raja, Plantain, Pome and Silk subgroups in the AAB group). Moreover, it appeared that Sanger sequencing of ITS could be used for identifying banana hybrid offspring. These results demonstrated that the banana ITS region contains rich genetic information that is useful for genotyping. In general, ITS sequencing simplifies the classification of banana germplasm resources and has potential application in several areas of Musa improvement.
Keywords
Musa; germplasm resources; genotypes; ITS
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
