Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Phenolic profiles and antitumor activity against colorectal cancer cells of seeds from selected Ribes taxa

Svetlana Lyashenko
ORCID logo ,
Rosalía López-Ruiz
,
Ana Minerva García-Cervantes
,
Ignacio Rodríguez-García
ORCID logo ,
Svetlana Yunusova
* ,
José Luis Guil-Guerrero
* ORCID logo
Version 1 : Received: 20 February 2024 / Approved: 20 February 2024 / Online: 20 February 2024 (14:28:22 CET)

A peer-reviewed article of this Preprint also exists.

Lyashenko, S.; López-Ruiz, R.; García-Cervantes, A.M.; Rodríguez-García, I.; Yunusova, S.; Guil-Guerrero, J.L. Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa. Appl. Sci. 2024, 14, 2428. Lyashenko, S.; López-Ruiz, R.; García-Cervantes, A.M.; Rodríguez-García, I.; Yunusova, S.; Guil-Guerrero, J.L. Phenolic Profiles and Antitumor Activity against Colorectal Cancer Cells of Seeds from Selected Ribes Taxa. Appl. Sci. 2024, 14, 2428.

Abstract

Seeds from several Ribes taxa were surveyed for phenolic compounds and in vitro antiproliferative activity against HT-29 colorectal cancer cells. Total phenolic compounds were analysed through the Folin-Ciocalteu procedure, while phenolic profiles were analysed by LC coupled to a single mass spectrometer Orbitrap using an electrospray interface (ESI). Antitumor effects were established using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Total phenolics ranged from 11.4 in R. alpinum to 94.8 mg of caffeic acid equivalents (CAE)/g in R. nigrum 'Koksa'. Concerning phenolic compounds, four were hydroxylated benzoic acids, four cinnamic acid derivatives, eight flavonoids, and nine flavonoid glycosides. The growth inhibition against HT-29 cancer cells was exercised much better by R. nigrum 'Koksa' and Ribes 'Erkeeni' (GI50 37 and 42 µg/ml). All Ribes extracts, except R. nigrum 'Hara katarlik', showed higher activity than that of R. rubrum (GI50 at 72 h: 99 µg/ml). Interestingly, the highly bioactive extract from Ribes 'Erkeeni' contains all detected phenolics, while R. nigrum 'Koksa' lacks only populnin. Therefore, the high bioactivity found for such extracts could be due to a synergy due to all detected compounds. This work constitutes a comprehensive action for expanding knowledge on the phenolic profiles and antitumor activity of GLA-rich Ribes seeds.

Keywords

Ribes; blackcurrant cultivars; phenolic compounds; LC-MS; HT-29; MTT assay

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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