Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

In Vivo-Matured Oocyte Resists Post-ovulatory Aging through the Post-transcriptional Regulation in Pigs

Cheng-Lin Zhan
,
Dongjie Zhou
,
Ming-Hong Sun
,
Wen-Jie Jiang
,
Song-Hee Lee
,
Xiao-Han Li
,
Qin-Yue Lu
,
Ji-Dam Kim
,
Gyu-Hyun Lee
,
Jae-Min Sim
,
Hak-Jae Chung
,
Eun-Seok Cho
,
Soo-Jin Sa
,
Xiang-Shun Cui
*
Version 1 : Received: 16 February 2024 / Approved: 19 February 2024 / Online: 19 February 2024 (16:01:18 CET)

How to cite: Zhan, C.; Zhou, D.; Sun, M.; Jiang, W.; Lee, S.; Li, X.; Lu, Q.; Kim, J.; Lee, G.; Sim, J.; Chung, H.; Cho, E.; Sa, S.; Cui, X. In Vivo-Matured Oocyte Resists Post-ovulatory Aging through the Post-transcriptional Regulation in Pigs. Preprints 2024, 2024020993. https://doi.org/10.20944/preprints202402.0993.v1 Zhan, C.; Zhou, D.; Sun, M.; Jiang, W.; Lee, S.; Li, X.; Lu, Q.; Kim, J.; Lee, G.; Sim, J.; Chung, H.; Cho, E.; Sa, S.; Cui, X. In Vivo-Matured Oocyte Resists Post-ovulatory Aging through the Post-transcriptional Regulation in Pigs. Preprints 2024, 2024020993. https://doi.org/10.20944/preprints202402.0993.v1

Abstract

Post-ovulatory aging (POA) is an inevitable factor during some assisted reproduction technology procedures, and results in poor fertilization rates and impaired embryo development. This study used RNA sequencing analysis and experimental validation to study the similarities and differences between in vivo- and in vitro-matured porcine oocytes before and after POA. Differentially expressed genes (DEGs) between fresh in vivo-matured oocyte (F_vivo) and aged in vivo-matured oocyte(A_vivo) and DEGs between fresh in vitro-matured oocyte (F_vitro) and aged in vitro-matured oocyte(A_vitro) were intersected to explore the co-effects of POA. It was found that "organelles", especially "mitochondria", were significantly enriched GO terms. The expression of genes related to the “electron transport chain” and “cell redox homeostasis” pathways related to mitochondrial function significantly showed low expression patterns in both A_vivo and A_vitro groups. WGCNA analysis to explore gene expression modules specific to A_vivo. Trait-modules association analysis showed that the red modules were most associated with in vivo aging. There are a total of 959 genes in the red module, which are mainly enriched in “RNA binding”, “mRNA metabolic process”, etc. GO terms, and “spliceosome” and “nucleotide excision repair” pathways. DNAJC7, IK, and DDX18 were at the hub of the gene regulatory network. And these genes were all expressed higher in A_vivo. In summary, POA affects the organelle function of oocytes. A_vivo oocytes have some unique gene expression patterns. These genes may be potential anti-aging targets. This study helps us understand the detailed mechanism of POA and provides strategies for researching oocyte aging.

Keywords

post-ovulatory aging; oocyte quality; mitochondrial function; post-transcriptional regulation

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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