Kim, Y.; Jo, M.; An, S.; Lee, Y.; Choi, E.D.; Jeong, M.-H.; Kim, K.-T.; Park, S.-Y. MoNOT3 Subunit Has Important Roles in Infection-Related Development and Stress Responses in Magnaporthe oryzae. Int. J. Mol. Sci.2024, 25, 3290.
Kim, Y.; Jo, M.; An, S.; Lee, Y.; Choi, E.D.; Jeong, M.-H.; Kim, K.-T.; Park, S.-Y. MoNOT3 Subunit Has Important Roles in Infection-Related Development and Stress Responses in Magnaporthe oryzae. Int. J. Mol. Sci. 2024, 25, 3290.
Kim, Y.; Jo, M.; An, S.; Lee, Y.; Choi, E.D.; Jeong, M.-H.; Kim, K.-T.; Park, S.-Y. MoNOT3 Subunit Has Important Roles in Infection-Related Development and Stress Responses in Magnaporthe oryzae. Int. J. Mol. Sci.2024, 25, 3290.
Kim, Y.; Jo, M.; An, S.; Lee, Y.; Choi, E.D.; Jeong, M.-H.; Kim, K.-T.; Park, S.-Y. MoNOT3 Subunit Has Important Roles in Infection-Related Development and Stress Responses in Magnaporthe oryzae. Int. J. Mol. Sci. 2024, 25, 3290.
Abstract
The multifunctional carbon-catabolite repression-negative on TATA box less complex (CCR4-NOT) is a multi-subunit complex present in all eukaryotes, including fungi. This complex plays an essential role in gene expression; however, a functional study of the CCR4-NOT complex in the rice blast fungus Magnaporthe oryzae has not been conducted. Seven genes encoding the putative CCR4-NOT complex were identified in the M. oryzae genome. Among these, a homologous gene, MoNOT3, was overexpressed during appressorium development in a previous study. Deletion of MoNOT3 in M. oryzae resulted in a significant reduction in hyphal growth, conidiation, abnormal septation in conidia, conidial germination, and appressorium formation compared to the wild-type. Transcriptional analyses suggested that the MoNOT3 gene affects conidiation and conidial morphology by regulating COS1 and COM1 in M. oryzae. Furthermore, monot3 exhibited a lack of pathogenicity, both with and without wounding, which is attributable to deficiencies in the development of invasive growth in planta. This result was also observed in onion epidermal cells, which are non-host plants. In addition, the MoNOT3 gene was involved in cell wall stress responses and heat shock. Taken together, these observations suggest that the MoNOT3 gene is required for fungal infection-related cell development and stress responses in M. oryzae.
Biology and Life Sciences, Agricultural Science and Agronomy
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