Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genetic Mapping and Characterization of Verticillium Wilt Resistance in a Recombinant Inbred Population of Upland Cotton

Iain W Wilson
* ORCID logo ,
Philippe Moncuquet
,
Yuman Yuan
,
Melanie Soliveres
ORCID logo ,
Zitong Li
,
Warwick Stiller
,
Qian-Hao Zhu
ORCID logo
Version 1 : Received: 30 January 2024 / Approved: 31 January 2024 / Online: 31 January 2024 (07:22:26 CET)

A peer-reviewed article of this Preprint also exists.

Wilson, I.W.; Moncuquet, P.; Yuan, Y.; Soliveres, M.; Li, Z.; Stiller, W.; Zhu, Q.-H. Genetic Mapping and Characterization of Verticillium Wilt Resistance in a Recombinant Inbred Population of Upland Cotton. Int. J. Mol. Sci. 2024, 25, 2439. Wilson, I.W.; Moncuquet, P.; Yuan, Y.; Soliveres, M.; Li, Z.; Stiller, W.; Zhu, Q.-H. Genetic Mapping and Characterization of Verticillium Wilt Resistance in a Recombinant Inbred Population of Upland Cotton. Int. J. Mol. Sci. 2024, 25, 2439.

Abstract

Verticillium wilt (VW) is an important and widespread disease of cotton and once established is long lived and difficult to manage. In Australia, the non-defoliating pathotype of Verticillium dahliae is the most common, and extremely virulent. Breeding cotton varieties with increased VW resistance is the most economical and effective method of controlling this disease and is greatly aided by understanding the genetics of resistance. In this study, VW resistance was investigated in 240 F7 recombinant inbred lines (RIL) derived from a cross between MCU-5 that has good resistance and Siokra 1-4 which is susceptible. Using a controlled environment bioassay, we found that resistance based on plant survival or shoot biomass was complex but with major contributions from chromosomes D03 and D09, with genomic prediction analysis estimating a prediction accuracy of 0.73 based on survival scores compared to 0.36 for shoot biomass. Transcriptome analysis of MCU-5 and Siokra 1-4 roots uninfected or infected with V. dahliae revealed that the two cultivars displayed very different root transcriptomes and responded differently to V. dahliae infection. Ninety-nine differentially expressed genes were located in the two mapped resistance regions and so are potential candidates for further identifying the genes responsible for VW resistance.

Keywords

Verticillium wilt; Disease resistance; Gossypium hirsutum; Verticillium dahliae; Cotton; Recombinant inbred lines

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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