Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A Pharmacological Investigation of the TMEM16A Currents in Murine Skeletal Myogenic Precursor Cells

Marina Sciancalepore
ORCID logo ,
Asja Ragnini
,
Paola Zacchi
,
Violetta Borelli
,
Paola D’Andrea
,
Paola Lorenzon
ORCID logo ,
Annalisa Bernareggi
* ORCID logo
Version 1 : Received: 18 January 2024 / Approved: 19 January 2024 / Online: 19 January 2024 (04:26:38 CET)

A peer-reviewed article of this Preprint also exists.

Sciancalepore, M.; Ragnini, A.; Zacchi, P.; Borelli, V.; D’Andrea, P.; Lorenzon, P.; Bernareggi, A. A Pharmacological Investigation of the TMEM16A Currents in Murine Skeletal Myogenic Precursor Cells. Int. J. Mol. Sci. 2024, 25, 2225. Sciancalepore, M.; Ragnini, A.; Zacchi, P.; Borelli, V.; D’Andrea, P.; Lorenzon, P.; Bernareggi, A. A Pharmacological Investigation of the TMEM16A Currents in Murine Skeletal Myogenic Precursor Cells. Int. J. Mol. Sci. 2024, 25, 2225.

Abstract

TMEM16A is a Ca2+-activated Cl- channel expressed in various species and tissues. In mammalian skeletal muscle precursors, the activity of these channels is still poorly investigated. Here, we characterized TMEM16A channels and investigated if the pharmacological activation of Piezo1 channels could modulate the TMEM16A currents in mouse myogenic precursors. Whole-cell patch-clamp recordings combined with the pharmacological agents Ani9, T16inh-A01 and Yoda1 were used to characterize TMEM16A-mediated currents and the possible modulatory effect of Piezo1 activity on TMEM16A channels. Western blot analysis was also carried out to confirm the expression of TMEM16A and Piezo1 channel proteins. We found that TMEM16A channels were functionally expressed in fusion-competent mouse myogenic precursors. The pharmacological blockage of TMEM16A inhibited myocyte fusion into myotubes. Moreover, the specific Piezo1 antagonist Yoda1 positively regulated TMEM16A currents. The findings demonstrate, for the first time, a sarcolemmal TMEM16A channel activity and its involvement at the early stage of mammalian skeletal muscle differentiation. In addition, the results suggest a possible role of mechanosensitive Piezo1 channels in the modulation of TMEM16A currents.

Keywords

TMEM16A; Piezo1; Ani9; TMEM16inh-A01; Yoda1; skeletal muscle; myogenesis

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.