Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Proteomic Profiling of Plasma and Gut-Derived Extracellular Vesicles in Obesity

Pedro Baptista Pereira
,
Estefa Torrejón
,
Inês Ferreira
,
Ana Sofia Carvalho
ORCID logo ,
Akiko Teshima
,
Inês Sousa-Lima
,
Hans Christian Beck
ORCID logo ,
Bruno Costa-Silva
,
Rune Matthiesen
ORCID logo ,
Maria Paula Macedo
* ORCID logo ,
Rita Machado Oliveria
*
Version 1 : Received: 9 January 2024 / Approved: 10 January 2024 / Online: 11 January 2024 (10:08:28 CET)

A peer-reviewed article of this Preprint also exists.

Baptista Pereira, P.; Torrejón, E.; Ferreira, I.; Carvalho, A.S.; Teshima, A.; Sousa-Lima, I.; Beck, H.C.; Costa-Silva, B.; Matthiesen, R.; Macedo, M.P.; de Oliveira, R.M. Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity. Nutrients 2024, 16, 736. Baptista Pereira, P.; Torrejón, E.; Ferreira, I.; Carvalho, A.S.; Teshima, A.; Sousa-Lima, I.; Beck, H.C.; Costa-Silva, B.; Matthiesen, R.; Macedo, M.P.; de Oliveira, R.M. Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity. Nutrients 2024, 16, 736.

Abstract

Obesity entails metabolic alterations across multiple organs, highlighting the role of inter-organ communication in its pathogenesis. Extracellular vesicles (EVs) are communication agents in physiological and pathological conditions and although they have been associated with obesi-ty-comorbidities, their protein cargo in this context remain largely unknown. To decipher the messages encapsulated in EVs, we isolated plasma derived EVs from diet induced obese murine models. Obese plasma EVs exhibited a decline in protein diversity while control EVs revealed significant enrichment in protein folding functions, highlighting the importance of proper folder in maintaining metabolic homeostasis. Previously, we revealed that gut derived EVs proteome holds particular significance in obesity. Here, we compared plasma and gut EVs and identified four proteins exclusively present in the control state of both EVs, revealing the potential for a non-invasive assessment of gut health by analyzing blood derived EVs. Given the relevance of post-translational modifications (PTMs), we observed a shift in chromatin-related proteins from glycation to acetylation in obese gut EVs, suggesting a regulatory mechanism targeting DNA transcription during obesity. This study provides valuable insights into novel roles of EVs and protein post-translational modifications in the intricate mechanisms underlying obesity, shed-ding light on potential biomarkers and pathways for future research.

Keywords

Extracellular Vesicles; Obesity; Prediabetes; Metabolism; Proteomics; Post-Translational Modifications; Acetylation; Glycation

Subject

Biology and Life Sciences, Endocrinology and Metabolism

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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