Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Plasma mir-203a-3p as a Novel Diagnostic Biomarker in Patients with Parkinson’s Disease Dementia

Ya-Fang Hsu
,
Shau-Ping Lin
ORCID logo ,
Yung-Tsai Chu
,
Yi-Tzang Tsai
,
Frederick Kin Hing Phoa
ORCID logo ,
Ruey-Meei Wu
* ORCID logo
Version 1 : Received: 4 January 2024 / Approved: 4 January 2024 / Online: 4 January 2024 (11:35:28 CET)
Version 2 : Received: 8 January 2024 / Approved: 8 January 2024 / Online: 9 January 2024 (14:48:40 CET)

A peer-reviewed article of this Preprint also exists.

Hsu, Y.-F.; Lin, S.-P.; Chu, Y.-T.; Tsai, Y.-T.; Huang, J.-W.; Phoa, F.K.H.; Wu, R.-M. Plasma miR-203a-3p as a Novel Predictor of Dementia in Patients with Parkinson’s Disease. Int. J. Mol. Sci. 2024, 25, 3554. Hsu, Y.-F.; Lin, S.-P.; Chu, Y.-T.; Tsai, Y.-T.; Huang, J.-W.; Phoa, F.K.H.; Wu, R.-M. Plasma miR-203a-3p as a Novel Predictor of Dementia in Patients with Parkinson’s Disease. Int. J. Mol. Sci. 2024, 25, 3554.

Abstract

The early detection of cognitive decline and timely non-pharmacological management or drug therapy are extremely important in providing care for Parkinson’s disease with dementia (PDD). In this study, we first conducted a discovery study to identify six plasma microRNAs that may allow for the differentiation of PD with or without mild cognitive impairment via NGS. A total of 120 participants were further recruited in a validation cohort and divided into four subgroups, namely, normal controls (HC), PD with no dementia (PDND), PD with mild cognitive impairment (PD-MCI) and PDD. Among the six candidates, miR-203a-3p was successfully detected in the plasma of the validation cohort using droplet digital PCR (ddPCR). Our results show that the ratio of miR-203a-3p/miR-16-5p observed in PDD was significantly increased compared to in PD-MCI (p < 0.001) and PDND (p = 0.041). Moreover, the ratio of miR-203a-3p/miR-16-5p showed a significant correlation with MoCA scores (r = -0.237, p = 0.024) in patients with PD (PwP). The ROC curve of the logistic regression model, consisting of the variables of age, the ratio of miR-203a-3p/miR-16-5p and the UPDRS III score, also demonstrated an average AUC of 0.883 via 5-fold cross-validation. In conclusion, the ratio of miR-203a-3p/miR-16-5p may serve as a potential biomarker for distinguishing cognitive dysfunction from PwP.

Keywords

Plasma Biomarker; MicroRNA; Parkinson’s Disease; Cognitive decline; Droplet digital PCR

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comments (1)

Comment 1
Received: 9 January 2024
Commenter: Ruey-Meei Wu
Commenter's Conflict of Interests: Author
Comment: Professional English editing, recommended by MDPI, was applied for this improved version.
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