Wang, Z.; Yin, M.; Han, J.; Wang, X.; Chang, J.; Ren, Z.; Wang, L. Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of ZIP Genes in Cucumber. Agriculture2024, 14, 133.
Wang, Z.; Yin, M.; Han, J.; Wang, X.; Chang, J.; Ren, Z.; Wang, L. Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of ZIP Genes in Cucumber. Agriculture 2024, 14, 133.
Wang, Z.; Yin, M.; Han, J.; Wang, X.; Chang, J.; Ren, Z.; Wang, L. Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of ZIP Genes in Cucumber. Agriculture2024, 14, 133.
Wang, Z.; Yin, M.; Han, J.; Wang, X.; Chang, J.; Ren, Z.; Wang, L. Pan-Genome-Wide Identification and Transcriptome-Wide Analysis of ZIP Genes in Cucumber. Agriculture 2024, 14, 133.
Abstract
The ZIPs (ZRT/IRT-like proteins) play critical roles in the absorption, transport, and intracellular balance of metal ions essential for various physiological processes in plants. However, little is known about the pan-genomic characteristics and properties of ZIP genes in cucumber (Cucumis sativus L.). In this study, we identified 10 CsZIP genes from the pan-genome of 13 C. sativus accessions. Among them, only CsZIP10 showed no variation in protein sequence length. We analyzed the gene structure, conserved domains, promoter cis-elements, and phylogenetic relationships of these 10 CsZIP genes derived from "9930". Based on phylogenetic analysis, the CsZIP genes were classified into three branches. Amino acid sequence comparison revealed the presence of conserved histidine residues in the ZIP proteins. Analysis of promoter cis-elements showed that most promoters contained elements responsive to plant hormones. Expression profiling in different tissues showed that most CsZIP genes were expressed at relatively low levels in C. sativus leave, stem, and tendril, and CsZIP7 and CsZIP10 were specifically expressed in root, indicating their potential involvement in the absorption and transport of metal ions. Transcriptomic data indicated that these 10 ZIP genes displayed responses to both downy mildew and powdery mildew, and CsZIP1 were significantly downregulated after both salt and heat treatments. In conclusion, this study deepens our understanding of the ZIP gene family and enhances our knowledge of the biological functions of CsZIP genes in C. sativus.
Keywords
pan-genome; Cucumis sativus; CsZIP
Subject
Biology and Life Sciences, Plant Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
