Puerariae Lobatae Radix Ameliorates Chronic Kidney Disease by Reshaping Gut Microbiota and Down-Regulating Wnt / β-Catenin Signaling

Peng Wu; Jingwen Xue; Zhangrui Zhu; Yao Yu; Qi Sun; Ming Xie; Benlin Wang; Pengcheng Huang; Zhengyuan Feng; Jie Zhao

doi:10.20944/preprints202401.0241.v2

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preprints.org > medicine and pharmacology > dietetics and nutrition > doi: 10.20944/preprints202401.0241.v2

Preprint Article Version 2 Preserved in Portico This version is not peer-reviewed

Puerariae Lobatae Radix Ameliorates Chronic Kidney Disease by Reshaping Gut Microbiota and Down-Regulating Wnt / β-Catenin Signaling

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Version 1 : Received: 3 January 2024 / Approved: 3 January 2024 / Online: 3 January 2024 (10:35:50 CET)
Version 2 : Received: 16 January 2024 / Approved: 17 January 2024 / Online: 17 January 2024 (11:11:19 CET)

How to cite: Wu, P.; Xue, J.; Zhu, Z.; Yu, Y.; Sun, Q.; Xie, M.; Wang, B.; Huang, P.; Feng, Z.; Zhao, J. Puerariae Lobatae Radix Ameliorates Chronic Kidney Disease by Reshaping Gut Microbiota and Down-Regulating Wnt / β-Catenin Signaling. Preprints 2024, 2024010241. https://doi.org/10.20944/preprints202401.0241.v2 Wu, P.; Xue, J.; Zhu, Z.; Yu, Y.; Sun, Q.; Xie, M.; Wang, B.; Huang, P.; Feng, Z.; Zhao, J. Puerariae Lobatae Radix Ameliorates Chronic Kidney Disease by Reshaping Gut Microbiota and Down-Regulating Wnt / β-Catenin Signaling. Preprints 2024, 2024010241. https://doi.org/10.20944/preprints202401.0241.v2

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MDPI and ACS Style

Wu, P.; Xue, J.; Zhu, Z.; Yu, Y.; Sun, Q.; Xie, M.; Wang, B.; Huang, P.; Feng, Z.; Zhao, J. Puerariae Lobatae Radix Ameliorates Chronic Kidney Disease by Reshaping Gut Microbiota and Down-Regulating Wnt / β-Catenin Signaling. Preprints 2024, 2024010241. https://doi.org/10.20944/preprints202401.0241.v2

APA Style

Wu, P., Xue, J., Zhu, Z., Yu, Y., Sun, Q., Xie, M., Wang, B., Huang, P., Feng, Z., & Zhao, J. (2024). Puerariae Lobatae Radix Ameliorates Chronic Kidney Disease by Reshaping Gut Microbiota and Down-Regulating Wnt / β-Catenin Signaling. Preprints. https://doi.org/10.20944/preprints202401.0241.v2

Chicago/Turabian Style

Wu, P., Zhengyuan Feng and Jie Zhao. 2024 "Puerariae Lobatae Radix Ameliorates Chronic Kidney Disease by Reshaping Gut Microbiota and Down-Regulating Wnt / β-Catenin Signaling" Preprints. https://doi.org/10.20944/preprints202401.0241.v2

Abstract

Gut microbiota dysfunction is a key factor affecting chronic kidney disease (CKD) susceptibility. Puerariae Lobatae Radix (PLR), a traditional Chinese medicine and food homologous herb, is known to promote gut microbiota homeostasis, however, its role in renoprotection remains unknown. This study aimed to investigate the efficacy and potential mechanism of PLR to alleviate CKD. An 8-week 2% NaCl-feeding murine model was applied to induce CKD and evaluate the therapeutic effect of PLR supplementary. After gavage for 8 weeks, PLR significantly alleviated CKD-associated creatinine, urine protein increasement and nephritic histopathological injury. Moreover, PLR protected kidney from fibrosis by reducing inflammatory response and down-regulating the canonical Wnt / β-catenin pathway. Further, PLR rescued gut microbiota dysbiosis and protected high salt-induced gut barrier dysfunction. We found enrichment of Akkermansia and Bifidobacterium after PLR intervention, the relative abundance of which were in positive correlation with normal maintenance of renal histology and function. Next, fecal microbiota transplantation experiment verified that the positive effect of PLR on CKD was at least partially exerted through gut microbiota reestablishment and down-regulation of the Wnt / β-catenin pathway. Our study provides evidence for new function of PLR on kidney protection and put forward a potential therapeutic strategy target for CKD.

Keywords

Puerariae Lobatae Radix; High salt diet; Chronic kidney disease; Wnt / β-catenin pathway; Gut microbiota; Fecal microbiota transplantation

Subject

Medicine and Pharmacology, Dietetics and Nutrition

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comment 1

Received: 17 January 2024
Commenter: Jingwen Xue
Commenter's Conflict of Interests: Author

Comment: We have revised the title and parts of the abstract.

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