Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Complete chloroplast genomes of Microula sikkimensis and comparative analyses with related species from Boraginaceae

Version 1 : Received: 28 December 2023 / Approved: 28 December 2023 / Online: 28 December 2023 (19:57:14 CET)

A peer-reviewed article of this Preprint also exists.

Gao, Y.; Chen, Z.; Li, X.; Malik, K.; Li, C. Comparative Analyses of Complete Chloroplast Genomes of Microula sikkimensis and Related Species of Boraginaceae. Genes 2024, 15, 226. Gao, Y.; Chen, Z.; Li, X.; Malik, K.; Li, C. Comparative Analyses of Complete Chloroplast Genomes of Microula sikkimensis and Related Species of Boraginaceae. Genes 2024, 15, 226.

Abstract

The present study provides a detailed analysis of the chloroplast genome of Microula sikkimensis. The genome was consisted of altogether 149,428bp and distinct regions, including large single-copy (81329bp), small single-copy (17261bp), and inverted repeat (25419bp). The genome contained 112 genes, including 78 protein-coding genes, 30 tRNA genes, and 4 rRNA genes, some exhibited duplication in the IR region. The chloroplast genome displayed different GC content across regions, with the IR region exhibiting the highest. Codon usage analysis and the identification of simple sequence repeats (SSRs) offer valuable genetic markers. Comparative analysis with other Boraginaceae species highlighted conservation and diversity in coding and noncoding regions. Phylogenetic analysis placed M. sikkimensis within the Boraginaceae family, revealing its distinct relationship with specific species.

Keywords

Microula sikkimensis; chloroplast genomes; comparative analysis; phylogenetic analysis; Boraginaceae

Subject

Biology and Life Sciences, Plant Sciences

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