Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Darlyara Reis Silva
,
Marion Nayon Braga Soares
,
Maria Cristina Rocha Silva
,
Mariana Costa Lima
,
Vitória Karla de Oliveira Silva-Moraes
ORCID logo ,
Givago Lopes Alves
,
Anyela Marcela Ríos-Ríos
,
Aldilene da Silva Lima
,
Marcos Vinícius Marques Pinheiro
,
Thais Roseli Corrêa
,
Diego Silva Batista
ORCID logo ,
Fábio Afonso Mazzei Moura de Assis Figueiredo
,
Fabrício de Oliveira Reis
ORCID logo ,
Tiago Massi Ferraz
ORCID logo ,
Sérgio Heitor Sousa Felipe
* ORCID logo
Version 1 : Received: 27 December 2023 / Approved: 27 December 2023 / Online: 28 December 2023 (09:04:25 CET)

A peer-reviewed article of this Preprint also exists.

Silva, D.R.; Soares, M.N.B.; Silva, M.C.R.; Lima, M.C.; Silva-Moraes, V.K.O.; Alves, G.L.; Ríos-Ríos, A.M.; Lima, A.S.; Pinheiro, M.V.M.; Corrêa, T.R.; Batista, D.S.; Figueiredo, F.A.M.M.A.; Reis, F.O.; Ferraz, T.M.; Felipe, S.H.S. Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization. Horticulturae 2024, 10, 107. Silva, D.R.; Soares, M.N.B.; Silva, M.C.R.; Lima, M.C.; Silva-Moraes, V.K.O.; Alves, G.L.; Ríos-Ríos, A.M.; Lima, A.S.; Pinheiro, M.V.M.; Corrêa, T.R.; Batista, D.S.; Figueiredo, F.A.M.M.A.; Reis, F.O.; Ferraz, T.M.; Felipe, S.H.S. Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization. Horticulturae 2024, 10, 107.

Abstract

Eryngium foetidum L., a biennial herb with diverse applications in food and traditional medicine, holds economic and pharmacological significance. Given its growing commercial interest, implementing biotechnological approaches like plant tissue culture is vital for sustainable propagation and metabolite production. In this study, we investigated the in vitro photoautotrophic potential of E. foetidum, examining growth, chlorophyll a fluorescence, photosynthetic pigments, and anatomical features under different sucrose concentrations and gas exchange rates. Acclimatization and survival rates of plants after ex vitro transfer were also assessed. E. foetidum exhibited robust growth in both photoautotrophic and photomixotrophic conditions, with natural ventilation significantly enhancing plant development. Chlorophyll a fluorescence and photosynthetic performance were influenced by sucrose and gas exchange, highlighting the importance of these factors in plant micropropagation. Moreover, the species demonstrated remarkable plasticity during acclimatization, with high survival rates and rapid inflorescence emission. The research provides valuable insights into optimizing in vitro cultivation conditions for E. foetidum, emphasizing the potential for large-scale clonal propagation and exploring secondary metabolites. The observed phenotypic plasticity underscores the adaptability of the species to diverse environments. These biotechnological strategies open avenues for future studies, including the application of elicitors for enhanced secondary metabolite production.

Keywords

culantro; gas exchange rate; natural ventilation; plant tissue culture; sucrose supplementation; wild coriander

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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