Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and Haemonchus contortus

Yonathan Tilahun
* ORCID logo ,
Jessica Quijada-Pinango
,
Felicia Johnson
,
Kayla Smith
,
Kristin Moore
,
Malcolm McCallum
,
Terry Gipson
,
Archana Yadav
,
Zaisen Wang
Version 1 : Received: 22 December 2023 / Approved: 25 December 2023 / Online: 25 December 2023 (04:48:30 CET)

How to cite: Tilahun, Y.; Quijada-Pinango, J.; Johnson, F.; Smith, K.; Moore, K.; McCallum, M.; Gipson, T.; Yadav, A.; Wang, Z. What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and Haemonchus contortus. Preprints 2023, 2023121796. https://doi.org/10.20944/preprints202312.1796.v1 Tilahun, Y.; Quijada-Pinango, J.; Johnson, F.; Smith, K.; Moore, K.; McCallum, M.; Gipson, T.; Yadav, A.; Wang, Z. What Metagenomics Reveals of Microbial Populations in a Host-Parasite Relationship Between Alpine Goats and Haemonchus contortus. Preprints 2023, 2023121796. https://doi.org/10.20944/preprints202312.1796.v1

Abstract

Abstract: Assessing infection by a parasite, such as Haemonchus contortus, requires time and experienced personnel. Field-based diagnostics using metagenomic species-specific targeting methods would be beneficial. Here, we examine two helminth control methods, zoledronic acid (ZA), an activator of γ δ T cells, and a neutralizing antibody (AB) against γ δ T cells. We conducted a randomized complete block designed investigation for the effects of Haemonchus contortus (Hc) infection on Alpine wethers (young castrated male goats) to identify if metagenomic methods will provide strong evidence for diagnostics. Different tissues were collected from wethers and the microbiome compositions were identified. The relative proportion of microbial community members in the environments; the richness of the microbial community; and the evenness of distribution in microbial community members were determined. It was hypothesized that numerous parameters that include many data points will provide a more precise means to identify infection or health based on similarity to uninfected control data parameters. We found that samples did not sequence well, when multiplexed with high concentration libraries, giving taxonomic numbers that had residual and unmeasured confounding bias related to analysis or selection that define significance of OTUs in the tissue samples at the phylum or species level.

Keywords

Keywords: metagenomic, gastrointestinal nematodes, microbial flora, Haemonchus contortus, host-parasite interaction

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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