Version 1
: Received: 22 December 2023 / Approved: 22 December 2023 / Online: 22 December 2023 (11:46:36 CET)
How to cite:
Zhang, W.; Yang, Q.; Song, Y.; Liu, W.; Li, Y. Exploratory Metabolomic Analysis for Characterizing the Metabolic Profile of the Urinary Bladder under Estrogen Deprivation. Preprints2023, 2023121748. https://doi.org/10.20944/preprints202312.1748.v1
Zhang, W.; Yang, Q.; Song, Y.; Liu, W.; Li, Y. Exploratory Metabolomic Analysis for Characterizing the Metabolic Profile of the Urinary Bladder under Estrogen Deprivation. Preprints 2023, 2023121748. https://doi.org/10.20944/preprints202312.1748.v1
Zhang, W.; Yang, Q.; Song, Y.; Liu, W.; Li, Y. Exploratory Metabolomic Analysis for Characterizing the Metabolic Profile of the Urinary Bladder under Estrogen Deprivation. Preprints2023, 2023121748. https://doi.org/10.20944/preprints202312.1748.v1
APA Style
Zhang, W., Yang, Q., Song, Y., Liu, W., & Li, Y. (2023). Exploratory Metabolomic Analysis for Characterizing the Metabolic Profile of the Urinary Bladder under Estrogen Deprivation. Preprints. https://doi.org/10.20944/preprints202312.1748.v1
Chicago/Turabian Style
Zhang, W., Wenheng Liu and Yao Li. 2023 "Exploratory Metabolomic Analysis for Characterizing the Metabolic Profile of the Urinary Bladder under Estrogen Deprivation" Preprints. https://doi.org/10.20944/preprints202312.1748.v1
Abstract
Background: Estrogen homeostasis is crucial for bladder function, and estrogen deprivation resulting from ovarian removal or ovarian dysfunction may lead to various bladder dysfunctions. However, the specific mechanisms are not fully understood. Methods: We simulated estrogen deprivation using a rat ovariectomy model and supplemented estrogen through subcutaneous injections. The metabolic characteristics of bladder tissue were analyzed using non-targeted metabolomics, followed by bioinformatics analysis to preliminarily reveal the association between estrogen deprivation and bladder function. Results: We successfully established a rat model with estrogen deprivation and, through multivariate analysis and validation, identified several promising biomarkers represented by 3, 5-tetradecadiencarnitine, lysoPC (15:0), and cortisol. Furthermore, we explored estrogen deprivation-related metabolic changes in the bladder primarily characterized by amino acid metabolism imbalance. Conclusions: This study, for the first time, depicted the metabolic profile of bladder resulting from estrogen deprivation, providing an important experimental basis for future research on bladder dysfunctions caused by estrogen deprivation.
Biology and Life Sciences, Endocrinology and Metabolism
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
