Park, J.; Kim, S.; Jo, M.; An, S.; Kim, Y.; Yoon, J.; Jeong, M.-H.; Kim, E.Y.; Choi, J.; Kim, Y.; Park, S.-Y. Isolation and Identification of Alternaria alternata from Potato Plants Affected by Leaf Spot Disease in Korea: Selection of Effective Fungicides. J. Fungi2024, 10, 53.
Park, J.; Kim, S.; Jo, M.; An, S.; Kim, Y.; Yoon, J.; Jeong, M.-H.; Kim, E.Y.; Choi, J.; Kim, Y.; Park, S.-Y. Isolation and Identification of Alternaria alternata from Potato Plants Affected by Leaf Spot Disease in Korea: Selection of Effective Fungicides. J. Fungi 2024, 10, 53.
Park, J.; Kim, S.; Jo, M.; An, S.; Kim, Y.; Yoon, J.; Jeong, M.-H.; Kim, E.Y.; Choi, J.; Kim, Y.; Park, S.-Y. Isolation and Identification of Alternaria alternata from Potato Plants Affected by Leaf Spot Disease in Korea: Selection of Effective Fungicides. J. Fungi2024, 10, 53.
Park, J.; Kim, S.; Jo, M.; An, S.; Kim, Y.; Yoon, J.; Jeong, M.-H.; Kim, E.Y.; Choi, J.; Kim, Y.; Park, S.-Y. Isolation and Identification of Alternaria alternata from Potato Plants Affected by Leaf Spot Disease in Korea: Selection of Effective Fungicides. J. Fungi 2024, 10, 53.
Abstract
Brown leaf spot disease caused by Alternaria spp. is among the most common diseases in potato crops. Typical brown spot symptoms were observed in commercial potato-cultivated areas of northern Korea during June 2021–2022. In total, 77 isolates were collected, and based on sequence analysis of the internal transcribed spacer (ITS) region, the collected isolates were identified as Alternaria spp. (83.1%). Phylogenetic analysis revealed that a majority of these isolates clustered within a clade that included A. alternata. Additionally, the ITS region and rpb2 yielded the most informative sequences for the identification of A. alternata. Pathogenicity tests confirmed that the collected pathogens elicited identical symptoms in the field. In the pathogenicity test performed on seven commercial cultivars, the pathogens exhibited strong virulence in both wound and non-wound inoculations. Among the cultivars tested, Arirang-1ho, Arirang-2ho, and Golden Ball were resistant to the pathogens. Furthermore, when testing effective fungicides in vitro, mancozeb and difenoconazole were found to be effective agents for inhibiting mycelial growth. In summary, our findings suggest that A. alternata plays a critical role in leaf disease in potato crop regions and emphasise the necessity for continuous monitoring and management to protect against this disease in South Korea.
Keywords
Alternaria alternata; brown spot disease; pathogenicity test; phylogenetic analysis; Solanum tuberosum L.
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
,
,
*
