Version 1
: Received: 19 December 2023 / Approved: 20 December 2023 / Online: 20 December 2023 (13:02:15 CET)
How to cite:
Luz, R.M.D.L.; Román, C.C.; Mar, R.L.D.; Jorge, C.; Marcos, S.R.; Lourdes, A.M.D.; Israel, C. Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants. Preprints2023, 2023121550. https://doi.org/10.20944/preprints202312.1550.v1
Luz, R.M.D.L.; Román, C.C.; Mar, R.L.D.; Jorge, C.; Marcos, S.R.; Lourdes, A.M.D.; Israel, C. Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants. Preprints 2023, 2023121550. https://doi.org/10.20944/preprints202312.1550.v1
Luz, R.M.D.L.; Román, C.C.; Mar, R.L.D.; Jorge, C.; Marcos, S.R.; Lourdes, A.M.D.; Israel, C. Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants. Preprints2023, 2023121550. https://doi.org/10.20944/preprints202312.1550.v1
APA Style
Luz, R.M.D.L., Román, C.C., Mar, R.L.D., Jorge, C., Marcos, S.R., Lourdes, A.M.D., & Israel, C. (2023). Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants. Preprints. https://doi.org/10.20944/preprints202312.1550.v1
Chicago/Turabian Style
Luz, R.M.D.L., Arévalo-Galarza Ma. de Lourdes and Castillo-Juárez Israel. 2023 "Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants" Preprints. https://doi.org/10.20944/preprints202312.1550.v1
Abstract
Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the multiplication of seedlings, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL-1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds, shoots, and seedling height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L-1 of BA and 0.1 mg L-1 of TDZ, as well as the MS base culture medium. With 0.1 mg L-1 of BA, 52-mm height, 1.36 shoots, and 9.22 buds were obtained, surpassing the MS control (MS culture medium alone); likewise, twice more roots (80%) and a 14% reduction in the bud structure were packed in 50 repetitions. The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L-1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. The results demonstrated that the induction of callogenesis in S. compositum has a better start from the stem in a medium with 2.0 mg L-1 of 2,4-D, whose value was 4.89 —equivalent to 97.8% callus formation around the explant. The addition of 500 mg L-1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callogenesis of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.
Keywords
agrobiodiversity; in vitro multiplication; Cucurbitaceae; underutilized species; Sechium (P. Br.)
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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