Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Recycled Brewer’s Spent Grains (BSG) and Grape Juice: A New Tool for Non-alcoholic (NAB) or Low Alcoholic (LAB) Craft Beer Using Non-conventional Yeasts

Version 1 : Received: 16 December 2023 / Approved: 18 December 2023 / Online: 18 December 2023 (10:43:06 CET)

A peer-reviewed article of this Preprint also exists.

Canonico, L.; Agarbati, A.; Comitini, F.; Ciani, M. Recycled Brewer’s Spent Grain (BSG) and Grape Juice: A New Tool for Non-Alcoholic (NAB) or Low-Alcoholic (LAB) Craft Beer Using Non-Conventional Yeasts. Foods 2024, 13, 505. Canonico, L.; Agarbati, A.; Comitini, F.; Ciani, M. Recycled Brewer’s Spent Grain (BSG) and Grape Juice: A New Tool for Non-Alcoholic (NAB) or Low-Alcoholic (LAB) Craft Beer Using Non-Conventional Yeasts. Foods 2024, 13, 505.

Abstract

Non-alcoholic beer (NAB) or low alcoholic beer (LAB) are taking over the market with a growing sale. Sus-tainable recycling and valorization of exhausted brewer’s spent grains (BSG) coming from craft beer is a relevant issue in brewing process. In this work, recycled BSG and BSG+GJ (supplemented with 10% grape juice) were used as wort-substrate to inoculate already selected non-conventional yeasts to produce NABLAB craft beer. Results showed that wort composed by only recycled BSG produced appreciate NAB beers (ethanol from 0.12% to 0.54 % v/v) while the addition of 10% of grape juice made LAB beers (ethanol from 0.82 to 1.66 % v/v). As expected, volatile compounds production was highest with the addition of grape juice. Lachancea thermotolerans confirmed the lactic acid production and, as Saccharomyces cerevisiae, is characterized by (in both worts) ethyl butyrate and isoamyl acetate, while Torulaspora delbrueckii produced relevant amounts of exanol, phenylethyl acetate and β-phenyl ethanol (wort added with grape juice). Wickeramhomyces anomalus and Pichia kluyvery showed consistent volatile production but only in wort with grape juice addition where a fermentation activity was exhibited. The overall results indicated that reused BSGs, non-conventional yeasts and grape juice is a suitable strategy for specialty NABLAB beer.

Keywords

non-alcoholic beer; low alcoholic beer; Italian Grape Ale; non-Saccharomyces; brewer’s spent grains

Subject

Biology and Life Sciences, Food Science and Technology

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