Constituents From Ageratina pichinchensis and Its Inhibitory Effect on Nitric Oxide Production

Mariana Sánchez-Ramos; Araceli Guerrero-Alonso; Antonio Romero-Estrada; Judith González-Christen; Laura Alvarez; Angélica Román-Guerrero; Francisco Cruz-Sosa; Silvia Marquina-Bahena

doi:10.20944/preprints202312.0814.v1

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preprints.org > biology and life sciences > biology and biotechnology > doi: 10.20944/preprints202312.0814.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Constituents From Ageratina pichinchensis and Its Inhibitory Effect on Nitric Oxide Production

Mariana Sánchez-Ramos

Araceli Guerrero-Alonso

Antonio Romero-Estrada

Judith González-Christen

Laura Alvarez

Angélica Román-Guerrero

Francisco Cruz-Sosa

^* ,

Silvia Marquina-Bahena

Version 1 : Received: 11 December 2023 / Approved: 12 December 2023 / Online: 12 December 2023 (07:20:53 CET)

How to cite: Sánchez-Ramos, M.; Guerrero-Alonso, A.; Romero-Estrada, A.; González-Christen, J.; Alvarez, L.; Román-Guerrero, A.; Cruz-Sosa, F.; Marquina-Bahena, S. Constituents From Ageratina pichinchensis and Its Inhibitory Effect on Nitric Oxide Production. Preprints 2023, 2023120814. https://doi.org/10.20944/preprints202312.0814.v1 Sánchez-Ramos, M.; Guerrero-Alonso, A.; Romero-Estrada, A.; González-Christen, J.; Alvarez, L.; Román-Guerrero, A.; Cruz-Sosa, F.; Marquina-Bahena, S. Constituents From Ageratina pichinchensis and Its Inhibitory Effect on Nitric Oxide Production. Preprints 2023, 2023120814. https://doi.org/10.20944/preprints202312.0814.v1

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MDPI and ACS Style

Sánchez-Ramos, M.; Guerrero-Alonso, A.; Romero-Estrada, A.; González-Christen, J.; Alvarez, L.; Román-Guerrero, A.; Cruz-Sosa, F.; Marquina-Bahena, S. Constituents From Ageratina pichinchensis and Its Inhibitory Effect on Nitric Oxide Production. Preprints 2023, 2023120814. https://doi.org/10.20944/preprints202312.0814.v1

APA Style

Sánchez-Ramos, M., Guerrero-Alonso, A., Romero-Estrada, A., González-Christen, J., Alvarez, L., Román-Guerrero, A., Cruz-Sosa, F., & Marquina-Bahena, S. (2023). Constituents From <em>Ageratina pichinchensis</em> and Its Inhibitory Effect on Nitric Oxide Production. Preprints. https://doi.org/10.20944/preprints202312.0814.v1

Chicago/Turabian Style

Sánchez-Ramos, M., Francisco Cruz-Sosa and Silvia Marquina-Bahena. 2023 "Constituents From <em>Ageratina pichinchensis</em> and Its Inhibitory Effect on Nitric Oxide Production" Preprints. https://doi.org/10.20944/preprints202312.0814.v1

Abstract

This work reports the isolation, purification, and anti-inflammatory evaluation of compounds from the plant species Ageratina pichinchesis. Using open-column chromatography, 11 known compounds were purified, whose chemical structures were elucidated by nuclear magnetic resonance techniques (1D and 2D). All compounds were evaluated in the in vitro model of RAW 264.7 mouse macrophage cells measuring nitric oxide inhibition as a determination of the anti-inflammatory effect. Outstandingly, the compound betuletol 3-O-β-glucoside (11) exhibited inhibition of nitric oxide with an IC50 of 75.08 ± 3.07% at 75 µM, additionally, it inhibited the secretion of interleukin 6 (IL-6) and activation of nuclear factor (NF-kβ). These results show that compound 11 could be considered as a potential anti-inflammatory agent in suppressing the expression of NF-kβ target genes such as the proinflammatory pathway and inducible nitric oxide synthase (iNOS). Consequently, the medicinal use attributed in traditional medicine to the A. pichinchensis species is due to the diversity of compounds that the plant biosynthesizes, demonstrating that its effect relates to anti-inflammatory effects associated with compound 11.

Keywords

betuletol 3-O-β-glucoside; A. pichinchensis; anti-inflammatory activity

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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