Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Alterations in Gene Expression during Incompatible Interaction between Amendoim Cavalo Common Bean and Colletotrichum lindemuthianum

Maike Lovatto
ORCID logo ,
Pedro Soares Vidigal Filho
* ,
Maria Celeste Gonçalves Vidigal
* ORCID logo ,
Mariana Vaz Bisneta
,
Alexandre Catto Calvi
,
Thiago Alexandre Santana Gilio
,
Eduardo Ansilieiro Nascimento
,
Maeli Melotto
Version 1 : Received: 8 December 2023 / Approved: 8 December 2023 / Online: 8 December 2023 (12:00:00 CET)

How to cite: Lovatto, M.; Vidigal Filho, P.S.; Gonçalves Vidigal, M.C.; Vaz Bisneta, M.; Calvi, A.C.; Gilio, T.A.S.; Nascimento, E.A.; Melotto, M. Alterations in Gene Expression during Incompatible Interaction between Amendoim Cavalo Common Bean and Colletotrichum lindemuthianum. Preprints 2023, 2023120609. https://doi.org/10.20944/preprints202312.0609.v1 Lovatto, M.; Vidigal Filho, P.S.; Gonçalves Vidigal, M.C.; Vaz Bisneta, M.; Calvi, A.C.; Gilio, T.A.S.; Nascimento, E.A.; Melotto, M. Alterations in Gene Expression during Incompatible Interaction between Amendoim Cavalo Common Bean and Colletotrichum lindemuthianum. Preprints 2023, 2023120609. https://doi.org/10.20944/preprints202312.0609.v1

Abstract

Anthracnose, caused by the fungus Colletotrichum lindemuthianum, poses a significant and widespread threat to the common bean crop. The use of plant genetic resistance has proven to be the most effective strategy for managing anthracnose disease. The Amendoim Cavalo (AC) Andean cultivar has resistance against multiple races of C. lindemuthianum, which is conferred by the Co-AC gene. Fine mapping of this resistance gene to common bean chromosome Pv01 enabled the identification of Phvul.001G244300, Phvul.001G244400, and Phvul.001G244500 candidate genes for further validation. In this study, we assessed the relative expression of Co-AC candidate genes, as well as other putative genes in vicinity of this locus and known resistance genes, in the AC cultivar following inoculation with the race 73 of C. lindemuthianum. Gene expression analysis revealed significantly higher expression levels of Phvul.001G244500. Notably, Phvul.001G244500 encodes a putative Basic Helix-Loop-Helix transcription factor, suggesting its involvement in the regulation of defense responses. Furthermore, we observed a significant modulation of the expression of defense related genes PR1a, PR1b, and PR2, in a time-course experiment. These findings contribute to the development of improved strategies for breeding anthracnose-resistant common bean cultivars, thereby mitigating the impact of this pathogen on crop yields and ensuring sustainable bean production.

Keywords

candidate gene expression; common bean–anthracnose interaction; plant defense genes

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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