Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Estimation of Genetic Diversity and Number of Unique Multilocus Genotypes (MLGs) of Cassava Germplasm from Burkina Faso Using Simple Sequence Repeat (SSR) Markers

Monique SORO
* ,
Serge Marie Felicien Wend-Pagnagdé Zida
ORCID logo ,
Koussao Somé
,
Fidèle Tiendrébéogo
* ORCID logo ,
Daniel H. Otron
,
Justin S. Pita
,
James B. Néya
,
Daouda Koné
Version 1 : Received: 4 December 2023 / Approved: 8 December 2023 / Online: 8 December 2023 (13:08:40 CET)

A peer-reviewed article of this Preprint also exists.

Soro, M.; Zida, S.M.-P.; Somé, K.; Tiendrébéogo, F.; Otron, D.H.; Pita, J.S.; Néya, J.B.; Koné, D. Estimation of Genetic Diversity and Number of Unique Genotypes of Cassava Germplasm from Burkina Faso Using Microsatellite Markers. Genes 2024, 15, 73. Soro, M.; Zida, S.M.-P.; Somé, K.; Tiendrébéogo, F.; Otron, D.H.; Pita, J.S.; Néya, J.B.; Koné, D. Estimation of Genetic Diversity and Number of Unique Genotypes of Cassava Germplasm from Burkina Faso Using Microsatellite Markers. Genes 2024, 15, 73.

Abstract

Genetic diversity is very important in crop improvement. This study was carried out to assess the genetic diversity and the number of unique multilocus genotypes (MLGs) in cassava collection in Burkina Faso. To achieve this objective 130 cassava accessions were genotyped using 32 simple sequence repeat (SSR) markers. The results revealed that among these markers, twelve (12) were highly informative, with the PIC values greater than 0.50, twelve (12) were moderately informative, with the PIC values ranged between 0.25 and 0.50, and eight (8) were not very informative, with the PIC values lower than 0.25. A moderate level of genetic diversity was found for the population, indicated by the average expected heterozygosity (0.45) and the observed heterozygosity (0.48). About 83.8% of unique multilocus genotypes were found in the cassava collection indicating that SSR markers seem to be most appropriate for MLGs identification. Population structure analysis based on hierarchical clustering identified two subpopulations and Bayesian approach suggested five clusters. Additionally, DAPC analysis separated the cassava accessions into 13 subpopulations. The comparison of these results and the previous study using SNP, suggest that each type of markers can be used to assessment the genetic structure of cassava grown in Burkina Faso.

Keywords

cassava, genotypes, genetic diversity, SSRs markers, population structure, Unique multilocus genotypes

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.