Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Human Serum Enriched After Absorption of Fish Cartilage Hydrolysate Enhances the Healing of Primary Human Dermal Fibroblasts Through the Expression of Proteins with Anti-Inflammatory and Immunomodulatory Properties

Julie Le Faouder
* ORCID logo ,
Aurelie Gueho
,
Regis Lavigne
,
Fabien Wauquier
,
Line Boutin-Wittrant
,
Elodie Bouvret
ORCID logo ,
Emmanuelle Com
,
Yohann Wittrant
ORCID logo ,
Charles Pineau
Version 1 : Received: 5 December 2023 / Approved: 6 December 2023 / Online: 8 December 2023 (07:53:19 CET)

How to cite: Le Faouder, J.; Gueho, A.; Lavigne, R.; Wauquier, F.; Boutin-Wittrant, L.; Bouvret, E.; Com, E.; Wittrant, Y.; Pineau, C. Human Serum Enriched After Absorption of Fish Cartilage Hydrolysate Enhances the Healing of Primary Human Dermal Fibroblasts Through the Expression of Proteins with Anti-Inflammatory and Immunomodulatory Properties. Preprints 2023, 2023120550. https://doi.org/10.20944/preprints202312.0550.v1 Le Faouder, J.; Gueho, A.; Lavigne, R.; Wauquier, F.; Boutin-Wittrant, L.; Bouvret, E.; Com, E.; Wittrant, Y.; Pineau, C. Human Serum Enriched After Absorption of Fish Cartilage Hydrolysate Enhances the Healing of Primary Human Dermal Fibroblasts Through the Expression of Proteins with Anti-Inflammatory and Immunomodulatory Properties. Preprints 2023, 2023120550. https://doi.org/10.20944/preprints202312.0550.v1

Abstract

Our skin epidermis constitutes the most important innate defense barrier against all pathogens. Therefore, even if skin lesions are common due to infections, scarring, genetic disorders, and other diseases, skin integrity is crucial for maintaining body homeostasis. Marine collagen peptides (MCPs) and Glycosaminoglycans (GAGs) have been described as potential wound healing (WH) agents. Recently, through an ex vivo clinical approach, we have demonstrated the nutricosmetic potential of fish cartilage hydrolysate (FCH), which presents a combination of collagen peptides and GAGs. Here, we coupled a clinical approach with in vitro experiments on human dermal fibroblasts (HDFs) and applied a Data Independent Acquisition - Parallel Accumulation Serial Fragmentation (diaPASEF) proteomic analysis, to assess the potential benefit of FCH in WH and its mode of action. Our results show that human serum enriched with circulating metabolites resulting from FCH ingestion, improved wound closure in scratch assays. In support, some proteins with anti-inflammatory and immunomodulatory properties or prone to promote hydration and extracellular matrix (ECM) stability showed increased expression in HDFs, after exposure to FCH-enriched serum. These findings suggest that FCH appears as a promising nutrient for WH and skin regeneration.

Keywords

skin health; nutraceuticals; marine hydrolyzed collagen; marine collagen peptides; chondroitin sulfate; glycosaminoglycans; proteomics; wound healing; primary cells; human dermal fibroblasts; clinical; ex vivo

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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