Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests Apolipoprotein A1 to Be a Potential Fertility Marker

Csilla Kurdi
,
János Schmidt
,
Zoltán Horváth-Szalai
ORCID logo ,
Péter Mauchart
,
Krisztina Gödöny
,
Ákos Várnagy
,
Gábor L Kovács
ORCID logo ,
Tamás Kőszegi
*
Version 1 : Received: 5 December 2023 / Approved: 6 December 2023 / Online: 6 December 2023 (07:44:23 CET)

A peer-reviewed article of this Preprint also exists.

Kurdi, C.; Schmidt, J.; Horváth-Szalai, Z.; Mauchart, P.; Gödöny, K.; Várnagy, Á.; Kovács, G.L.; Kőszegi, T. Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests That Apolipoprotein A1 Is a Potential Fertility Marker. Int. J. Mol. Sci. 2024, 25, 486. Kurdi, C.; Schmidt, J.; Horváth-Szalai, Z.; Mauchart, P.; Gödöny, K.; Várnagy, Á.; Kovács, G.L.; Kőszegi, T. Follicular Fluid Proteomic Analysis of Women Undergoing Assisted Reproduction Suggests That Apolipoprotein A1 Is a Potential Fertility Marker. Int. J. Mol. Sci. 2024, 25, 486.

Abstract

Infertility affects millions worldwide, posing a significant global health challenge. The proteomic analysis of follicular fluid provides a comprehensive view of the complex molecular landscape within ovarian follicles, offering valuable information on the factors influencing oocyte development and on the overall reproductive health. The follicular fluid is derived from the plasma and contains various proteins that can have different roles in oocyte health and infertility and this fluid is a critical microenvironment for the developing oocytes as well. Using HPLC/MS method we investigated the protein composition of the follicular fluid and after classification we did relative quantification of the identified proteins in the pregnant (P) and non-pregnant (NP) groups. Based on the protein-protein interaction analysis ALB and ApoA1 was found to be hub proteins and the quantitative comparison of the P and NP groups resulted significantly lower concentration of ApoA1 and HDL-cholesterol in the P group. As both molecules are involved in the cholesterol transport, we also investigated their role in the development of oocytes and in the prediction of fertility.

Keywords

assisted reproductive treatment; follicular fluid; proteomics; mass spectrometry; apolipoprotein A1; HDL cholesterol; predictive value

Subject

Medicine and Pharmacology, Obstetrics and Gynaecology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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