Version 1
: Received: 5 December 2023 / Approved: 6 December 2023 / Online: 6 December 2023 (04:03:43 CET)
How to cite:
Kuehnel, H.; Pasztorek, M.; Kuten-Pella, O.; Kramer, K.; Bauer, C.; Lacza, Z.; Nehrer, S. Effect of Blood-Derived Products on Senescence Associated Se-Cretory Phenotype in an Etoposide Induced Senescence Human Dermal Fibroblast Model. Preprints2023, 2023120304. https://doi.org/10.20944/preprints202312.0304.v1
Kuehnel, H.; Pasztorek, M.; Kuten-Pella, O.; Kramer, K.; Bauer, C.; Lacza, Z.; Nehrer, S. Effect of Blood-Derived Products on Senescence Associated Se-Cretory Phenotype in an Etoposide Induced Senescence Human Dermal Fibroblast Model. Preprints 2023, 2023120304. https://doi.org/10.20944/preprints202312.0304.v1
Kuehnel, H.; Pasztorek, M.; Kuten-Pella, O.; Kramer, K.; Bauer, C.; Lacza, Z.; Nehrer, S. Effect of Blood-Derived Products on Senescence Associated Se-Cretory Phenotype in an Etoposide Induced Senescence Human Dermal Fibroblast Model. Preprints2023, 2023120304. https://doi.org/10.20944/preprints202312.0304.v1
APA Style
Kuehnel, H., Pasztorek, M., Kuten-Pella, O., Kramer, K., Bauer, C., Lacza, Z., & Nehrer, S. (2023). Effect of Blood-Derived Products on Senescence Associated Se-Cretory Phenotype in an Etoposide Induced Senescence Human Dermal Fibroblast Model. Preprints. https://doi.org/10.20944/preprints202312.0304.v1
Chicago/Turabian Style
Kuehnel, H., Zsombor Lacza and Stefen Nehrer. 2023 "Effect of Blood-Derived Products on Senescence Associated Se-Cretory Phenotype in an Etoposide Induced Senescence Human Dermal Fibroblast Model" Preprints. https://doi.org/10.20944/preprints202312.0304.v1
Abstract
Blood-derived products (BP) like citrate-platelet-rich plasma (CPRP) or hyperacute serum (HAS) are known to contain many growth factors. Combined mitogenic and DNA damaging stimuli might lead to increased senescent cell burden and altered senescence-associated secretory pheno-type (SASP). Therefore, the senescent state was extensively tested by γH2AX staining, p21 Q-PCR and western blot, growth curves and senescence associated ß-galactosidase staining. Two main treatments with BP were performed early (immediately after etoposide) and late (after addition-al 11days). Effects of the BP treatment was evaluated by IL-6 and IL-8 measurement as well as collagen (COL1) and p21 mRNA expression. Additionally, XTT assays, cell size measurements, viability assays, and cell number calculations were performed. HAS treated cells in early treat-ment had the lowest observed IL-6 and IL-8. In contrast, there was a clear inflammatory response for IL-8 on HAS and CPRP treated cells in late treatment. For COL1 expression an upregulation in early treatment could be shown, meanwhile cells in the late treatment group remained unaf-fected. In CPRP treated cells, the COL1 expression decreased. All in all, BP treatment seems to have slightly positive effects regarding skin rejuvenation.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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