Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Clinical Application of the Association Between Genetic Alteration and Intraoperative Fluorescence Activity of 5-Aminolevulinic Acid During the Resection of Brain Metastasis of Lung Adenocarcinoma

Hyeon Yeong Jeong
,
Won Jun Suh
,
Kim Seung Hwan
ORCID logo ,
Nam Taek Min
,
Ji Hwan Jang
,
Kim Kyu Hong
,
Seok Hyun Kim
,
Young Zoon Kim
* ORCID logo
Version 1 : Received: 4 December 2023 / Approved: 4 December 2023 / Online: 5 December 2023 (04:44:19 CET)

A peer-reviewed article of this Preprint also exists.

Jeong, H.Y.; Suh, W.J.; Kim, S.H.; Nam, T.M.; Jang, J.H.; Kim, K.H.; Kim, S.H.; Kim, Y.Z. Clinical Application of the Association between Genetic Alteration and Intraoperative Fluorescence Activity of 5-Aminolevulinic Acid during the Resection of Brain Metastasis of Lung Adenocarcinoma. Cancers 2024, 16, 88. Jeong, H.Y.; Suh, W.J.; Kim, S.H.; Nam, T.M.; Jang, J.H.; Kim, K.H.; Kim, S.H.; Kim, Y.Z. Clinical Application of the Association between Genetic Alteration and Intraoperative Fluorescence Activity of 5-Aminolevulinic Acid during the Resection of Brain Metastasis of Lung Adenocarcinoma. Cancers 2024, 16, 88.

Abstract

: The primary objective of this study was to investigate the association of certain genetic alterations and intraoperative fluorescent activity of 5-aminolevulinic acid (ALA) in brain metastasis (BM) of lung adenocarcinoma. A retrospective cohort study was conducted among 72 patients who underwent surgical resection of BM from lung adenocarcinoma at our institute for 5 years. Cancer cell infiltration was estimated by the intraoperative fluorescent activity of 5-ALA, and genetic alterations were analyzed by next-generation sequencing (NGS). The sensitivity and specificity for detecting cancer cell infiltration using 5-ALA were 87.5% and 96.4%, respectively. Genes associated with cell cycle regulation (p = 0.003) and cell proliferation (p = 0.044) were significantly associated with positive fluorescence activity of 5-ALA in the adjacent brain tissue. Genetic alterations in cell cycle regulation and cell proliferation were also associated with shorter recurrence-free survival (p = 0.013 and p = 0.042, respectively) and overall survival (p = 0.026 and p = 0.042, respectively) in the multivariate analysis. The results suggest that genetic alterations in cell cycle regulation and cell proliferation are associated with positive fluorescence activity of 5-ALA in the adjacent infiltrative brain tissue and influence the clinical outcome of BM of lung adenocarcinoma.

Keywords

brain metastasis; lung cancer; 5-aminolevulinic acid; genetic alteration; next-generation sequencing; infiltration; cell cycle regulation; proliferation

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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