Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Green Synthesis, Characterization and Antibacterial Activity of Gold and Copper Nanoparticles from Lannea discolor (Sond.) Engl

Version 1 : Received: 30 November 2023 / Approved: 1 December 2023 / Online: 1 December 2023 (13:36:53 CET)

A peer-reviewed article of this Preprint also exists.

Rambau, U.; Masevhe, N.A.; Samie, A. Green Synthesis of Gold and Copper Nanoparticles by Lannea discolor: Characterization and Antibacterial Activity. Inorganics 2024, 12, 36. Rambau, U.; Masevhe, N.A.; Samie, A. Green Synthesis of Gold and Copper Nanoparticles by Lannea discolor: Characterization and Antibacterial Activity. Inorganics 2024, 12, 36.

Abstract

Green synthesis using plant extracts has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. The leaf, stem, and root extracts of Lannea discolor was utilized as a reducing and stabilizing agent in the synthesis of gold (AuNPs) and copper (CuNPs) nanoparticles. The formation of AuNPs and CuNPs confirmed by their color change, was characterised by UV–Vis Spectroscopy (UV- Vis), Scanning electron microscopy analysis and energy dispersive x-ray (SEM-EDX), Transmission Electron Microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FTIR), coupled with minimum concentration inhibitory (MIC) antibacterial assays. AuNFs, NPs and CuNPs peaked at wavelengths in the ranges of 316, 544 and 564 nm respectively. TEM showed unexpected nanoflowers (30 – 97 nm) in the leaf extracts and spherical NPs (10 -33 nm; 9.3 – 37.5) from stem and root extracts, while spherical CuNPs (20 – 104 nm), were observed from all the extracts. EDX was able to confirm the presence of metal salts and FTIR revealed stable capping agents. AuNPs and NFs from L. discolor extracts showed appreciable antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus subtilis, when compared to the plant extracts, while none was observed from the CuNPs. These AuNPs and CuNPs are particularly appealing in a variety of applications in the biomedical as well as conductivity manufacturing, due to their shapes and sizes and economical and environmentally friendly production.

Keywords

copper nanoparticles; gold nanoflowers; green nanoparticle synthesis; Lannea discolor; plant extracts

Subject

Biology and Life Sciences, Plant Sciences

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