Garrido-Godino, A.I.; Gupta, I.; Pelechano, V.; Navarro, F. RNA Pol II Assembly Affects ncRNA Expression. Int. J. Mol. Sci.2024, 25, 507.
Garrido-Godino, A.I.; Gupta, I.; Pelechano, V.; Navarro, F. RNA Pol II Assembly Affects ncRNA Expression. Int. J. Mol. Sci. 2024, 25, 507.
Garrido-Godino, A.I.; Gupta, I.; Pelechano, V.; Navarro, F. RNA Pol II Assembly Affects ncRNA Expression. Int. J. Mol. Sci.2024, 25, 507.
Garrido-Godino, A.I.; Gupta, I.; Pelechano, V.; Navarro, F. RNA Pol II Assembly Affects ncRNA Expression. Int. J. Mol. Sci. 2024, 25, 507.
Abstract
RNA pol II assembly occurs in the cytoplasm before the translocation of the enzyme to the nucleus. Affecting assembly influences mRNA transcription in the nucleus and mRNA decay in the cytoplasm. However, very little is known about the consequences on ncRNA synthesis. In this work, we show that impairment of RNA pol II assembly leads to a decrease in cryptic non-coding RNAs (preferentially CUTs and SUTs). This alteration is partially restored upon overcoming the assembly defect. Notably, this drop in ncRNAs is only partially dependent on the nuclear exosome, which suggests a major specific effect of enzyme assembly. Our data also point out a defect in transcription termination and lead as to proposes that CTD phosphatase Rtr1 could be involved in this process.
Biology and Life Sciences, Biochemistry and Molecular Biology
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