Version 1
: Received: 17 November 2023 / Approved: 17 November 2023 / Online: 17 November 2023 (14:26:34 CET)
How to cite:
Reyes Castillo, N.; Díaz, C.E.; Andres, M.F.; Imperial, J.; Varcacel, F.; González Coloma, A. Optimization of Fungicidal and Acaricidal Metabolite Production by Aspergillus sp. SPH2. Preprints2023, 2023111175. https://doi.org/10.20944/preprints202311.1175.v1
Reyes Castillo, N.; Díaz, C.E.; Andres, M.F.; Imperial, J.; Varcacel, F.; González Coloma, A. Optimization of Fungicidal and Acaricidal Metabolite Production by Aspergillus sp. SPH2. Preprints 2023, 2023111175. https://doi.org/10.20944/preprints202311.1175.v1
Reyes Castillo, N.; Díaz, C.E.; Andres, M.F.; Imperial, J.; Varcacel, F.; González Coloma, A. Optimization of Fungicidal and Acaricidal Metabolite Production by Aspergillus sp. SPH2. Preprints2023, 2023111175. https://doi.org/10.20944/preprints202311.1175.v1
APA Style
Reyes Castillo, N., Díaz, C.E., Andres, M.F., Imperial, J., Varcacel, F., & González Coloma, A. (2023). Optimization of Fungicidal and Acaricidal Metabolite Production by Aspergillus sp. SPH2. Preprints. https://doi.org/10.20944/preprints202311.1175.v1
Chicago/Turabian Style
Reyes Castillo, N., Félix Varcacel and Azucena González Coloma. 2023 "Optimization of Fungicidal and Acaricidal Metabolite Production by Aspergillus sp. SPH2" Preprints. https://doi.org/10.20944/preprints202311.1175.v1
Abstract
The endophytic fungus Aspergillus sp. SPH2 was isolated from the stems of the endemic plant Bethencourtia palmensis and its extracts were found to have strong fungicidal effects against Botrytis cinerea and ixodicidal effects against Hyalomma lusitanicum at different fermentation times. In this study, the fungus was grown using three different culture media and two methodologies, Microparticulate Enhancement Cultivation (MPEC) and Semi-Solid Fermentation (SSF), to increase the production of secondary metabolites in submerged fermentation. The addition of an inert support to the culture medium (SSF) resulted in a significant increase in extract production. However, when talcum powder was added to different culture media, unexpected results were observed, with a decrease in the production of the biocompounds of interest. Metabolomic analyses showed that the production of aspergillic, neoaspergillic, and neohydroxyaspergillic acids peaked in the first few days of fermentation, with notable differences observed among the methodologies and culture media. Mellein production was particularly affected by the addition of an inert support to the culture medium. These results highlight the importance of surface properties and morphology of spores and mycelia during fermentation by this fungal species.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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